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Study Information
Study Attribute Value
CEBS Accession Number: 002-01561-0006-0000-1
Chemical Name: 7,12-Dimethylbenzanthracene
CASRN: 57-97-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G13734
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 106 57 23
    131r 135 63 16
Test Chemical 7,12-Dimethylbenzanthracene 
12.5       
 112 97 56 17 20
    98 45 66 22
  
25         
 108 70 70 22
    147r 108 71 16
  
50         
 92 29 66 24 25
    93 51 72 26
  
100         
 60 14 48 27 39
    34 10 53 51
Positive Control Ethyl Methane Sulfonate 
500         
 49 19 813 557 618*
    42 17 850 680
Negative Control Negative (Not Specified) 
 0         
 107 106 57 18 21*
    78 88 58 25

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 97 101 64 22 22
    96 78 65 23
Test Chemical 7,12-Dimethylbenzanthracene 
12.5       
 86 85 78 30 28
    83 80 65 26
  
25         
 95 75 71 25 26
    97# 74 79 27
  
50         
 94 47 88 31 31
    95 48 90 32
  
100         
 50 4 56 38 42*
    56 7 78 46
Positive Control Ethyl Methane Sulfonate 
500         
 65# 40 977 499 516*
    67 40 1072 533
Negative Control Negative (Not Specified) 
 0         
 94 120 70 25 27*
    93 101 81 29

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 57 111 32 19 17
    84 88 36 14
    61 101 32 18
    47r 65 19 14
Test Chemical 7,12-Dimethylbenzanthracene 
2.5       
 49 72 28 19 16
    54 85 26 16
    62 76 25 14
  
5         
 58 94 9 5 10
    82 97 27 11
    57 111 24 14
  
10         
 52 109 20 13 13
    101 138 31 10
    70 138 30 14
  
20         
 64 103 39 20 14
    73 89 18 8
    61 91 23 13
  
40         
 49 111 16 11 13
    56 96 23 14
    72 112 29 13
  
60         
 59 90 24 14 13
    59 110 27 15
    64 113 19 10
Positive Control Ethyl Methane Sulfonate 
500         
 32 35 437 455 378*
    39 66 413 356
    48 56 461 322

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 114 89 98 29 29
    106 111 92 29
Test Chemical 7,12-Dimethylbenzanthracene 
 0.625     
 97 63 258 89 96*
    91 37 282 103
  
1.25      
 95 37 410 144 138*
    81 55 322 133
  
2.5       
 72 19 454 210 218*
    59 18 401 227
  
5         
 50 17 379 253 258*
    52 23 415 264
  
10         
 75 16 460 204 217*
    59 32 410 230
Positive Control 3-Methylcholanthrene 
5         
 102 62 356 116 147*
    90 29 476 177
Negative Control Negative (Not Specified) 
 0         
 128r 202 94 25
    127r 175 75 20

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 120 86 29 31
    81 88 101 41
    88# 92 66 25
    91 100 82 30
Test Chemical 7,12-Dimethylbenzanthracene 
 0.313     
 81 85 146 60 57*
    91# 94 145 53
  
 0.625     
 72 43 164 76 81*
    76 73 198 87
  
1.25      
 67 65 203 102 124*
    54# 49 235.5 146
  
2.5       
 47 31 277 196 194*
    51# 22 297 193
  
5         
    9r 1 76 298
  
10         
   
Positive Control 3-Methylcholanthrene 
5         
 14 4 375 872 590*
    34 11 312 307

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 75 104 44 69
    97 124 138 48
    81 101 228 94
    67 100 183 91
Test Chemical 7,12-Dimethylbenzanthracene 
 0.156     
 101 64 327 108 102
    67 93 193 96
  
 0.313     
 67 71 266 133 141*
    59 71 263 148
  
 0.625     
 69 86 381 185 175*
    69 69 346 166
  
1.25      
 73 49 483 222 211*
    71 62 429 201
  
2.5       
 34 11 460 447 390*
    50 16 496 333
Positive Control 3-Methylcholanthrene 
5         
 38 10 455 403 372*
    43 13 443 341

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination