Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01561-0006-0000-1
Chemical Name:	7,12-Dimethylbenzanthracene
CASRN:	57-97-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G13734
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	106	57	23
Vehicle Control			131r	135	63	16
Test Chemical	7,12-Dimethylbenzanthracene	12.5	112	97	56	17	20
			98	45	66	22	20
		25	108	70	70	22
			147r	108	71	16
		50	92	29	66	24	25
			93	51	72	26	25
		100	60	14	48	27	39
			34	10	53	51	39
Positive Control	Ethyl Methane Sulfonate	500	49	19	813	557	618*
Positive Control			42	17	850	680	618*
Negative Control	Negative (Not Specified)	0	107	106	57	18	21*
Negative Control			78	88	58	25	21*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	101	64	22	22
Vehicle Control			96	78	65	23	22
Test Chemical	7,12-Dimethylbenzanthracene	12.5	86	85	78	30	28
			83	80	65	26	28
		25	95	75	71	25	26
			97#	74	79	27	26
		50	94	47	88	31	31
			95	48	90	32	31
		100	50	4	56	38	42*
			56	7	78	46	42*
Positive Control	Ethyl Methane Sulfonate	500	65#	40	977	499	516*
Positive Control			67	40	1072	533	516*
Negative Control	Negative (Not Specified)	0	94	120	70	25	27*
Negative Control			93	101	81	29	27*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	57	111	32	19	17
			84	88	36	14
			61	101	32	18
			47r	65	19	14
Test Chemical	7,12-Dimethylbenzanthracene	2.5	49	72	28	19	16
			54	85	26	16
			62	76	25	14
		5	58	94	9	5	10
			82	97	27	11
			57	111	24	14
		10	52	109	20	13	13
			101	138	31	10
			70	138	30	14
		20	64	103	39	20	14
			73	89	18	8
			61	91	23	13
		40	49	111	16	11	13
			56	96	23	14
			72	112	29	13
		60	59	90	24	14	13
			59	110	27	15
			64	113	19	10
Positive Control	Ethyl Methane Sulfonate	500	32	35	437	455	378*
			39	66	413	356
			48	56	461	322

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	114	89	98	29	29
Vehicle Control			106	111	92	29	29
Test Chemical	7,12-Dimethylbenzanthracene	0.625	97	63	258	89	96*
			91	37	282	103	96*
		1.25	95	37	410	144	138*
			81	55	322	133	138*
		2.5	72	19	454	210	218*
			59	18	401	227	218*
		5	50	17	379	253	258*
			52	23	415	264	258*
		10	75	16	460	204	217*
			59	32	410	230	217*
Positive Control	3-Methylcholanthrene	5	102	62	356	116	147*
Positive Control			90	29	476	177	147*
Negative Control	Negative (Not Specified)	0	128r	202	94	25
Negative Control			127r	175	75	20

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	120	86	29	31
			81	88	101	41
			88#	92	66	25
			91	100	82	30
Test Chemical	7,12-Dimethylbenzanthracene	0.313	81	85	146	60	57*
			91#	94	145	53	57*
		0.625	72	43	164	76	81*
			76	73	198	87	81*
		1.25	67	65	203	102	124*
			54#	49	235.5	146	124*
		2.5	47	31	277	196	194*
			51#	22	297	193	194*
		5
			9r	1	76	298
		10

Positive Control	3-Methylcholanthrene	5	14	4	375	872	590*
Positive Control			34	11	312	307	590*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	75	104	44	69
			97	124	138	48
			81	101	228	94
			67	100	183	91
Test Chemical	7,12-Dimethylbenzanthracene	0.156	101	64	327	108	102
			67	93	193	96	102
		0.313	67	71	266	133	141*
			59	71	263	148	141*
		0.625	69	86	381	185	175*
			69	69	346	166	175*
		1.25	73	49	483	222	211*
			71	62	429	201	211*
		2.5	34	11	460	447	390*
			50	16	496	333	390*
Positive Control	3-Methylcholanthrene	5	38	10	455	403	372*
Positive Control			43	13	443	341	372*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination