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Study Information
Study Attribute Value
CEBS Accession Number: 002-01561-0007-0000-2
Chemical Name: 7,12-Dimethylbenzanthracene
CASRN: 57-97-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G58149
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 85 82 30 30
    107 115 97 30
Test Chemical 7,12-Dimethylbenzanthracene 
29.4       
 94 164 68 24 31
    53 80 60 38
  
42         
 93 88 103 37 41
    84 93 115 46
  
60         
 104r 66 117 38
  
85.8       
 50r 9 68 45
  
123         
 8r 1 28 114
    15r 1 19 42
  
250         
   
Positive Control Ethyl Methane Sulfonate 
500         
 54 33 968 596 533*
    75 45 1059 471

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 104 158 56 63
    92 96 193 70
Test Chemical 7,12-Dimethylbenzanthracene 
42         
 111 93 135 41 36
    92 81 87 31
  
49         
 95 74 113 40 38
    102 77 110 36
  
57         
 93 50 136 49 55
    91 51 168 61
  
66         
 91 20 142 52 66
    92 22 219 80
  
77         
 43 4 350 269 212*
    40 3 188 155
  
89         
   
Positive Control Ethyl Methane Sulfonate 
500         
 67 54 883 437 431*
    71 54 898 425

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 101 81 31 37
    81 99 101 42
    82 95 90 37
    89 105 100 37
Test Chemical 7,12-Dimethylbenzanthracene 
16.8       
 83 102 134 54 48
    105 122 132 42
    78 85 115 49
  
24         
 85 88 125 49 46
    107 91 139 43
    95 103 127 45
  
34.3       
 75 77 108 48 52
    94 60 157 55
    95 93 148 52
  
49         
 92 86 140 51 42
    85 84 102 40
    96 94 101 35
  
70         
 96 77 122 42 48
    108 87 167 52
    78 76 119 51
  
100         
 63 4 192 101 132*
    45 3 218 163
Positive Control Ethyl Methane Sulfonate 
500         
 62 42 1069 578 612*
    51 39 1010 658
    60 40 1075 599

Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 96 405 131 128
    97 104 367 126
Test Chemical 7,12-Dimethylbenzanthracene 
1.31      
 93 85 437 157 152
    109 98 476 146
  
1.64      
 93 86 355 127 132
    89 78 363 136
  
2.05      
 85 71 354 139 160
    78 68 425 182
  
2.56      
 84 68 375 149 163
    70 55 373 177
  
3.2       
 68 40 404 197 191
    75 47 416 184
  
4         
 50 22 406 270 270*
    48 18 388 270
  
5         
 15 2 302 686 611*
    17 3 265 535
Positive Control 3-Methylcholanthrene 
5         
 41 17 517 426 406*
    43 18 492 386

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 100 257 91 94
    81 100 236 97
Test Chemical 7,12-Dimethylbenzanthracene 
1.05      
 92 105 279 101 112
    93 98 341 123
  
1.31      
 77 87 293 127 116
    101# 105 321 106
  
1.64      
 82 87 411 168 176*
    90 95 499 184
  
2.05      
 93 91 460 164 178*
    69 66 399 192
  
2.56      
 73 65 430 196 193*
    86 93 491 190
  
3.2       
 66 60 494 249 296*
    50 49 514 343
  
4         
 52 41 579 369
  
5         
 31 15 354 387 495*
    28 12 509 602
Positive Control 3-Methylcholanthrene 
5         
 40 27 418 345 361*
    38 22 434 377

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination