Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01561-0007-0000-2
Chemical Name:	7,12-Dimethylbenzanthracene
CASRN:	57-97-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G58149
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	85	82	30	30
Vehicle Control			107	115	97	30	30
Test Chemical	7,12-Dimethylbenzanthracene	29.4	94	164	68	24	31
			53	80	60	38	31
		42	93	88	103	37	41
			84	93	115	46	41
		60	104r	66	117	38
		85.8	50r	9	68	45
		123	8r	1	28	114
			15r	1	19	42
		250

Positive Control	Ethyl Methane Sulfonate	500	54	33	968	596	533*
Positive Control			75	45	1059	471	533*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	104	158	56	63
Vehicle Control			92	96	193	70	63
Test Chemical	7,12-Dimethylbenzanthracene	42	111	93	135	41	36
			92	81	87	31	36
		49	95	74	113	40	38
			102	77	110	36	38
		57	93	50	136	49	55
			91	51	168	61	55
		66	91	20	142	52	66
			92	22	219	80	66
		77	43	4	350	269	212*
			40	3	188	155	212*
		89

Positive Control	Ethyl Methane Sulfonate	500	67	54	883	437	431*
Positive Control			71	54	898	425	431*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	101	81	31	37
			81	99	101	42
			82	95	90	37
			89	105	100	37
Test Chemical	7,12-Dimethylbenzanthracene	16.8	83	102	134	54	48
			105	122	132	42
			78	85	115	49
		24	85	88	125	49	46
			107	91	139	43
			95	103	127	45
		34.3	75	77	108	48	52
			94	60	157	55
			95	93	148	52
		49	92	86	140	51	42
			85	84	102	40
			96	94	101	35
		70	96	77	122	42	48
			108	87	167	52
			78	76	119	51
		100	63	4	192	101	132*
			45	3	218	163	132*
Positive Control	Ethyl Methane Sulfonate	500	62	42	1069	578	612*
			51	39	1010	658
			60	40	1075	599

Induced S9 Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	96	405	131	128
Vehicle Control			97	104	367	126	128
Test Chemical	7,12-Dimethylbenzanthracene	1.31	93	85	437	157	152
			109	98	476	146	152
		1.64	93	86	355	127	132
			89	78	363	136	132
		2.05	85	71	354	139	160
			78	68	425	182	160
		2.56	84	68	375	149	163
			70	55	373	177	163
		3.2	68	40	404	197	191
			75	47	416	184	191
		4	50	22	406	270	270*
			48	18	388	270	270*
		5	15	2	302	686	611*
			17	3	265	535	611*
Positive Control	3-Methylcholanthrene	5	41	17	517	426	406*
Positive Control			43	18	492	386	406*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	100	257	91	94
Vehicle Control			81	100	236	97	94
Test Chemical	7,12-Dimethylbenzanthracene	1.05	92	105	279	101	112
			93	98	341	123	112
		1.31	77	87	293	127	116
			101#	105	321	106	116
		1.64	82	87	411	168	176*
			90	95	499	184	176*
		2.05	93	91	460	164	178*
			69	66	399	192	178*
		2.56	73	65	430	196	193*
			86	93	491	190	193*
		3.2	66	60	494	249	296*
			50	49	514	343	296*
		4	52	41	579	369
		5	31	15	354	387	495*
			28	12	509	602	495*
Positive Control	3-Methylcholanthrene	5	40	27	418	345	361*
Positive Control			38	22	434	377	361*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination