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Study Information
Study Attribute Value
CEBS Accession Number: 002-01563-0002-0000-9
Chemical Name: 7-Bromomethyl-12-methylbenz(a)anthracene
CASRN: 16238-56-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G55517
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 111 98 44 48
    84 89 130 51
Test Chemical 7-Bromomethyl-12-methylbenz(a)anthracene 
 0.03      
 71 95 223 105 89*
    50 89 110 73
  
 0.06      
 47 71 168 120 119*
    53 81 186 117
  
 0.13      
 49 57 254 173 145*
    60 79 209 116
  
 0.25      
 54 58 320 196 202*
    48 42 296 207
  
 0.5       
 23 10 329 484 608*
    12 3 271 732
  
1         
  
2         
   
Positive Control Ethyl Methane Sulfonate 
500         
 11 8 560 1723 1204*
    28 19 576 686

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 98 93 30 34
    108 102 121 38
Test Chemical 7-Bromomethyl-12-methylbenz(a)anthracene 
 0.016     
 108 97 201 62 62*
    117 106 216 62
  
 0.031     
 103 86 289 93
    148r 129 342 77
  
 0.063     
 98 79 298 101 139*
    73 66 385 176
  
 0.125     
 82 49 622 254 217*
    87 65 470 180
  
 0.25      
 47 14 676 478 402*
    67 35 661 327
  
 0.5       
 14 1 254 620 785*
    14 2 385 951
  
1         
   
Positive Control Ethyl Methane Sulfonate 
500         
 63 35 963 508 502*
    62 37 919 497

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 90 205 84 77
    83 110 176 71
Test Chemical 7-Bromomethyl-12-methylbenz(a)anthracene 
 0.06      
 69 92 211 102 98
    85 105 242 95
  
 0.13      
 57 71 291 169 154*
    76 85 315 139
  
 0.25      
 67 73 364 182 170*
    69 70 328 158
  
 0.5       
 61 54 446 245 248*
    55 51 409 250
  
1         
 37 22 508 462 435*
    36 22 435 408
  
2         
 7r 2 188 940
    8r 2 156 624
  
4         
   
Positive Control 3-Methylcholanthrene 
7         
 45 40 462 343 294*
    57 48 420 245

Non-Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 93 134 63 61
    95 107 167 58
Test Chemical 7-Bromomethyl-12-methylbenz(a)anthracene 
 0.13      
 82 102 338 137 134*
    89 108 351 131
  
 0.25      
 78 78 363 156 155*
    78 96 359 153
  
 0.5       
 73 71 406 186 213*
    53 48 380 239
  
1         
 50 28 441 295 310*
    46 27 449 324
  
2         
 35 11 444 429 469*
    31 10 479 510
Positive Control 3-Methylcholanthrene 
7         
 66 33 438 221 271*
    50 18 476 321
3-Methylcholanthrene 
10         
 8r 4 126 548
    5r 3 102 703

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 113 100 36 44
    95 87 151 53
Test Chemical 7-Bromomethyl-12-methylbenz(a)anthracene 
 0.67      
 73 75 237 108 98*
    49 53 130 88
  
 0.96      
 72 68 315 146 115*
    81 84 206 85
  
1.4       
 76 63 368 161 148*
    82 76 335 136
  
2         
 62 41 396 212 198*
    66 43 364 184
  
2.8       
 44 19 361 276 267*
    45 17 344 258
  
4         
 28 6 347 408 482*
    28 7 462 557
Positive Control 3-Methylcholanthrene 
5         
 75 75 414 184 197*
    69 69 434 211
3-Methylcholanthrene 
100         
 6r 3 103 624
    9r 6 129 487

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 101 141 59 48
    82 99 92 37
Test Chemical 7-Bromomethyl-12-methylbenz(a)anthracene 
 0.47      
 77 78 263 114 105*
    68 64 194 96
  
 0.67      
 79 80 193 82 87*
    77 90 212 92
  
 0.96      
 71 83 230 109 106*
    80# 100 250.5 104
  
1.4       
 80 80 208 87 98*
    69 68 224 109
  
2         
 69 68 379 184 159*
    67 65 269 133
  
2.8       
 54 40 359 221 193*
    78 66 387 165
Positive Control 3-Methylcholanthrene 
5         
 41 34 467 377 304*
    57 51 392 231
3-Methylcholanthrene 
100         
 6r 4 82 432
    6r 4 53 321

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination