Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01563-0002-0000-9
Chemical Name:	7-Bromomethyl-12-methylbenz(a)anthracene
CASRN:	16238-56-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G55517
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	111	98	44	48
Vehicle Control			84	89	130	51	48
Test Chemical	7-Bromomethyl-12-methylbenz(a)anthracene	0.03	71	95	223	105	89*
			50	89	110	73	89*
		0.06	47	71	168	120	119*
			53	81	186	117	119*
		0.13	49	57	254	173	145*
			60	79	209	116	145*
		0.25	54	58	320	196	202*
			48	42	296	207	202*
		0.5	23	10	329	484	608*
			12	3	271	732	608*
		1
		2

Positive Control	Ethyl Methane Sulfonate	500	11	8	560	1723	1204*
Positive Control			28	19	576	686	1204*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	98	93	30	34
Vehicle Control			108	102	121	38	34
Test Chemical	7-Bromomethyl-12-methylbenz(a)anthracene	0.016	108	97	201	62	62*
			117	106	216	62	62*
		0.031	103	86	289	93
			148r	129	342	77
		0.063	98	79	298	101	139*
			73	66	385	176	139*
		0.125	82	49	622	254	217*
			87	65	470	180	217*
		0.25	47	14	676	478	402*
			67	35	661	327	402*
		0.5	14	1	254	620	785*
			14	2	385	951	785*
		1

Positive Control	Ethyl Methane Sulfonate	500	63	35	963	508	502*
Positive Control			62	37	919	497	502*

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	90	205	84	77
Vehicle Control			83	110	176	71	77
Test Chemical	7-Bromomethyl-12-methylbenz(a)anthracene	0.06	69	92	211	102	98
			85	105	242	95	98
		0.13	57	71	291	169	154*
			76	85	315	139	154*
		0.25	67	73	364	182	170*
			69	70	328	158	170*
		0.5	61	54	446	245	248*
			55	51	409	250	248*
		1	37	22	508	462	435*
			36	22	435	408	435*
		2	7r	2	188	940
			8r	2	156	624
		4

Positive Control	3-Methylcholanthrene	7	45	40	462	343	294*
Positive Control			57	48	420	245	294*

Non-Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	93	134	63	61
Vehicle Control			95	107	167	58	61
Test Chemical	7-Bromomethyl-12-methylbenz(a)anthracene	0.13	82	102	338	137	134*
			89	108	351	131	134*
		0.25	78	78	363	156	155*
			78	96	359	153	155*
		0.5	73	71	406	186	213*
			53	48	380	239	213*
		1	50	28	441	295	310*
			46	27	449	324	310*
		2	35	11	444	429	469*
			31	10	479	510	469*
Positive Control	3-Methylcholanthrene	7	66	33	438	221	271*
			50	18	476	321	271*
	3-Methylcholanthrene	10	8r	4	126	548
			5r	3	102	703

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	113	100	36	44
Vehicle Control			95	87	151	53	44
Test Chemical	7-Bromomethyl-12-methylbenz(a)anthracene	0.67	73	75	237	108	98*
			49	53	130	88	98*
		0.96	72	68	315	146	115*
			81	84	206	85	115*
		1.4	76	63	368	161	148*
			82	76	335	136	148*
		2	62	41	396	212	198*
			66	43	364	184	198*
		2.8	44	19	361	276	267*
			45	17	344	258	267*
		4	28	6	347	408	482*
			28	7	462	557	482*
Positive Control	3-Methylcholanthrene	5	75	75	414	184	197*
			69	69	434	211	197*
	3-Methylcholanthrene	100	6r	3	103	624
			9r	6	129	487

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	101	141	59	48
Vehicle Control			82	99	92	37	48
Test Chemical	7-Bromomethyl-12-methylbenz(a)anthracene	0.47	77	78	263	114	105*
			68	64	194	96	105*
		0.67	79	80	193	82	87*
			77	90	212	92	87*
		0.96	71	83	230	109	106*
			80#	100	250.5	104	106*
		1.4	80	80	208	87	98*
			69	68	224	109	98*
		2	69	68	379	184	159*
			67	65	269	133	159*
		2.8	54	40	359	221	193*
			78	66	387	165	193*
Positive Control	3-Methylcholanthrene	5	41	34	467	377	304*
			57	51	392	231	304*
	3-Methylcholanthrene	100	6r	4	82	432
			6r	4	53	321

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination