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Study Information
Study Attribute Value
CEBS Accession Number: 002-01571-0001-0000-7
Chemical Name: 9-Aminoacridine, monohydrochloride, monohydrate
CASRN: 52417-22-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 686905_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Positive 
No Activation  3  Test Failure 
Induced Rat Liver S9  1_S9  Test Failure 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 12/13/1983    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 537 0.512 10.74 26.0 0.000
Test Chemical 9-Aminoacridine, monohydrochloride, monohydrate 
 0.083     
 0 0 0 0.000 0.00 32.2 N/A 
 0.25      
 50 1052 891 0.847 17.82 32.2 65.291 
 0.833     
 0 0 0 0.000 0.00 32.2 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1053 894 0.849 17.88 26.0 65.690
Mitomycin-C 
 0.01      
 5 105 227 2.162 45.40 26.0 321.914
Trend: 9.296        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 02/26/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 528 0.504 10.56 26.0 0.000
Test Chemical 9-Aminoacridine, monohydrochloride, monohydrate 
 0.35      
 50 1045 753 0.721 15.06 26.0 42.887 
 0.4       
 50 1047 806 0.770 16.12 26.0 52.652 
 0.5       
 50 1049 790 0.753 15.80 26.0 49.336
Positive Control Mitomycin-C 
 0.0015    
 50 1049 855 0.815 17.10 26.0 61.623
Mitomycin-C 
 0.01      
 5 105 236 2.248 47.20 26.0 345.693
Trend: 6.722        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 03/08/1984    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 525 219 0.417 8.76 26.0 0.000
Test Chemical 9-Aminoacridine, monohydrochloride, monohydrate 
1         
 0 0 0 0.000 0.00 35.5 N/A 
1.25      
 25 534 521 0.976 20.84 35.5 133.890 
1.25      
 25 1037 1062 1.024 42.48 35.5 145.505 
1.5       
 0 0 0 0.000 0.00 35.5 N/A
Positive Control Mitomycin-C 
 0.0015    
 0 0 0 0.000 0.00 0.0 N/A
Mitomycin-C 
 0.01      
 0 0 0 0.000 0.00 0.0 N/A
Trend: 12.408        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/13/1983    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 603 0.579 12.06 26.0 0.000
Test Chemical 9-Aminoacridine, monohydrochloride, monohydrate 
2.5       
 50 1049 609 0.581 12.18 26.0 0.321 
8.33      
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1041 801 0.769 16.02 26.0 32.963
Cyclophosphamide 
2         
 5 105 212 2.019 42.40 26.0 248.897
Trend: 0.056        
Probability: 0.478        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/26/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 679 0.647 13.58 26.0 0.000
Test Chemical 9-Aminoacridine, monohydrochloride, monohydrate 
10         
 50 1049 887 0.846 17.74 34.0 30.758 
15         
 50 1046 964 0.922 19.28 34.0 42.516 
20         
 25 522 507 0.971 20.28 34.0 50.196 
25         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1050 945 0.900 18.90 26.0 39.175
Cyclophosphamide 
2         
 5 105 237 2.257 47.40 26.0 249.043
Trend: 7.650        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 03/08/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 525 230 0.438 9.20 26.0 0.000
Test Chemical 9-Aminoacridine, monohydrochloride, monohydrate 
10         
 25 525 361 0.688 14.44 26.0 56.957 
15         
 25 526 450 0.856 18.00 35.5 95.280 
20         
 25 528 507 0.960 20.28 35.5 119.182 
25         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 25 524 414 0.790 16.56 26.0 80.344
Cyclophosphamide 
2         
 5 105 302 2.876 60.40 26.0 556.522
Trend: 10.363        
Probability: 0.000