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Study Information
Study Attribute Value
CEBS Accession Number: 002-01573-0001-0000-9
Chemical Name: AF-2
CASRN: 3688-53-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 655476_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 08/15/1980    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1020 335 0.328 6.70 26.0 0.000
Negative (Not Specified) 
0         
 50 1028 343 0.334 6.86 26.0 1.591
Test Chemical AF-2 
 0.003     
 50 1033 508 0.492 10.16 26.0 49.733 
 0.016     
 35 733 362 0.494 10.34 26.0 50.370 
 0.08      
 50 1029 508 0.494 10.16 26.0 50.315 
 0.4       
 50 1034 833 0.806 16.66 26.0 145.290 
2         
 50 1048 1336 1.275 26.72 28.3 288.151
Positive Control Triethylenemelamine 
 0.02      
 50 1044 2085 1.997 41.70 26.0 508.080
Trend: 26.735        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 08/26/1980    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1040 384 0.369 7.68 26.0 4.265
Dimethyl Sulfoxide 
0         
 50 1042 369 0.354 7.38 26.0 0.000
Test Chemical AF-2 
 0.23      
 50 1043 399 0.383 7.98 26.0 8.026 
 0.95      
 50 1041 376 0.361 7.52 26.0 1.995 
3.75      
 50 1043 395 0.379 7.90 26.0 6.943 
15         
 50 1046 356 0.340 7.12 26.0 -3.892 
60         
 50 1041 518 0.498 10.36 26.0 40.514 
240         
 7 145 230 1.586 32.86 28.0 347.921
Positive Control Cyclophosphamide 
1.5       
 50 1042 2249 2.158 44.98 26.0 509.485
Trend: 10.019        
Probability: 0.000