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Study Information
Study Attribute Value
CEBS Accession Number: 002-01573-0003-0000-1
Chemical Name: AF-2
CASRN: 3688-53-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 658476_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/14/1980    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 381 0.364 7.62 26.0 0.000
Test Chemical AF-2 
 0.005     
 50 1042 447 0.429 8.94 26.0 17.773 
 0.05      
 50 1046 440 0.421 8.80 26.0 15.486 
 0.5       
 50 1046 602 0.576 12.04 26.0 58.005
Positive Control Triethylenemelamine 
 0.025     
 50 1051 2248 2.139 44.96 26.0 487.219
Trend: 6.763        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 08/13/1980    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 337 0.322 6.74 26.0 0.000
Test Chemical AF-2 
 0.005     
 50 1045 467 0.447 9.34 26.0 38.841 
 0.05      
 50 1048 534 0.510 10.68 26.0 58.306 
 0.5       
 50 1049 692 0.660 13.84 26.0 104.950
Positive Control Triethylenemelamine 
 0.025     
 50 1046 2157 2.062 43.14 26.0 540.671
Trend: 11.190        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 01/28/1981    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 423 0.403 8.46 26.0 0.000
Test Chemical AF-2 
 0.016     
 50 1050 430 0.410 8.60 26.0 1.655 
 0.05      
 50 1050 480 0.457 9.60 26.0 13.475 
 0.16      
 50 1050 565 0.538 11.30 26.0 33.570 
 0.5       
 50 1050 669 0.637 13.38 26.0 58.156 
1.6       
 31 629 589 0.936 19.00 26.0 132.441
Positive Control Triethylenemelamine 
 0.025     
 50 1050 1896 1.806 37.92 26.0 348.227
Trend: 15.124        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/14/1980    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 405 0.387 8.10 26.0 0.000
Test Chemical AF-2 
 0.005     
 50 1046 388 0.371 7.76 26.0 -4.106 
 0.05      
 50 1046 365 0.349 7.30 26.0 -9.790 
 0.5       
 50 1046 362 0.346 7.24 26.0 -10.532 
5         
 50 1046 418 0.400 8.36 26.0 3.309 
50         
 50 1046 641 0.613 12.82 26.0 58.423
Positive Control Cyclophosphamide 
1.5       
 50 1047 1757 1.678 35.14 26.0 333.827
Trend: 7.315        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/15/1981    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 384 0.366 7.68 26.0 0.000
Test Chemical AF-2 
1.6       
 50 1050 415 0.395 8.30 26.0 8.073 
5         
 50 1050 427 0.407 8.54 26.0 11.198 
16         
 50 1050 444 0.423 8.88 26.0 15.625 
50         
 50 1048 614 0.586 12.28 28.3 60.201 
160         
 50 1048 1059 1.010 21.18 28.3 176.308
Positive Control Cyclophosphamide 
1.5       
 50 1049 1656 1.579 33.12 26.0 331.661
Trend: 20.264        
Probability: 0.000