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Study Information
Study Attribute Value
CEBS Accession Number: 002-01573-0004-0000-2
Chemical Name: AF-2
CASRN: 3688-53-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 658476_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 08/13/1980    Harvest Time: 14 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: AF-2 
 0.005     
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
 0.05      
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
 0.5       
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
5         
 100 25 0.250 19.0 9 0.090 9.0 16 0.160 14.0 0 0.000 0.0
Positive Control: Triethylenemelamine 
 0.25      
 100 58 0.580 38.0 24 0.240 22.0 33 0.330 24.0 1 0.010 1.0
Trend: 5.840 3.926 5.093  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 07/14/1980    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 8 0.080 8.0 2 0.020 2.0 6 0.060 6.0 0 0.000 0.0
Test Chemical: AF-2 
 0.005     
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0 
 0.05      
 100 15 0.150 14.0 1 0.010 1.0 14 0.140 14.0 0 0.000 0.0 
 0.5       
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 20 0.200 16.0 2 0.020 2.0 18 0.180 16.0 0 0.000 0.0 
50         
 100 7 0.070 7.0 2 0.020 2.0 5 0.050 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
25         
 100 122 1.220 67.0 52 0.520 38.0 66 0.660 43.0 4 0.040 4.0
Trend: 0.752 0.171 0.953  
Probability: 0.226 0.432 0.170  


Trial #:2   Activation: No Activation    Date: 07/14/1980    Harvest Time: 14 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: AF-2 
 0.005     
 100 5 0.050 1.0 0 0.000 0.0 5 0.050 1.0 0 0.000 0.0 
 0.05      
 100 15 0.150 15.0 5 0.050 5.0 10 0.100 10.0 0 0.000 0.0 
 0.5       
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 22 0.220 18.0 7 0.070 7.0 15 0.150 14.0 0 0.000 0.0
Positive Control: Triethylenemelamine 
 0.25      
 100 33 0.330 26.0 8 0.080 8.0 24 0.240 17.0 1 0.010 1.0
Trend: 3.866 2.893 3.301  
Probability: 0.000 0.002 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/15/1981    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: AF-2 
 0.16      
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
 0.5       
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0 
1.6       
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
5         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
16         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
50         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
160         
 100 5 0.050 4.0 2 0.020 2.0 3 0.030 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
25         
 100 53 0.530 36.0 11 0.110 10.0 42 0.420 30.0 0 0.000 0.0
Trend: 1.300 1.857 0.183  
Probability: 0.097 0.032 0.427  


Trial #:3   Activation: No Activation    Date: 01/15/1981    Harvest Time: 14 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: AF-2 
 0.16      
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
 0.5       
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
1.6       
 100 5 0.050 4.0 4 0.040 4.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 8 0.080 5.0 4 0.040 4.0 4 0.040 2.0 0 0.000 0.0 
16         
 100 17 0.170 14.0 6 0.060 5.0 10 0.100 10.0 1 0.010 1.0
Positive Control: Triethylenemelamine 
 0.25      
 100 35 0.350 27.0 14 0.140 11.0 21 0.210 18.0 0 0.000 0.0
Trend: 4.538 3.168 3.534  
Probability: 0.000 0.001 0.000