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Study Information
Study Attribute Value
CEBS Accession Number: 002-01592-0008-0000-7
Chemical Name: Acetohexamide
CASRN: 968-81-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 677347
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 61 75 31 44
    59 85 121 69
    90 113 62 23
    78# 141 122 52
Test Chemical Acetohexamide 
250         
 115 117 112 33 42
    94 91 131 47
    94 89 132 47
  
500         
 118 89 102 29 32
    94 102 115 41
    104 102 85 27
  
750         
 108 73 102 32 27
    119 89 103 29
    115 102 72 21
  
1000         
 125r 81 198 53 33
    119 66 142 40
    117 91 89 25
  
1500         
 128r 64 161 42
    126r 72 137 36
    126r 75 130 35
  
2000         
 90 20 177 66 219*
    86 17 204 79
    11 3 169 512
Positive Control Methyl Methane Sulfonate 
5         
 42 28 245 195 271*
    56 40 334 199
    39# 29 485 418

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 94 131 40 34
    115 102 120 35
    115 104 99 29
    121r 97 150 41
Test Chemical Acetohexamide 
250         
 116 101 141 41 32
    108 84 93 29
    114 94 91 27
  
500         
 115 74 117 34 35
    110 81 117 35
    162r 140 159 33
  
750         
 106 77 94 30 29
    117 93 71 20
    109 91 118 36
  
1000         
 124r 76 208 56
    121r 89 88 24
    112r 70 109 32
  
1500         
 108 93 173 54 57*
    111 80 251 76
    116# 66 147 42
  
2000         
 116 79 160 46 49
    99 60 157 53
    121r 74 240 66
Positive Control Methyl Methane Sulfonate 
5         
 113 74 596 176 246*
    105# 58 722 229
    82 52 812 332

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 96 94 44 41
    72 100 84 39
    119 104 98 27
    66 99 104 53
Test Chemical Acetohexamide 
125         
 72 84 78 36 49
    45 67 85 63
  
250         
 60 69 94 52 68*
    56 63 126 76
    54 67 122 75
  
500         
 63# 74 85.5 46 38
    77 77 88 38
    110 94 99 30
  
750         
 46 46 104 76 80*
    46 50 138 100
    55 57 104 63
  
1000         
 78 85 111 47 52
    80 79 110 46
    57 56 106 62
  
1500         
 81 95 99 41 41
    78 89 72 31
    77 73 118 51
Positive Control 3-Methylcholanthrene 
2.5       
 64 59 356 186 222*
    54 36 381 234
    48 44 353 247

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 113 111 220 65 54
    106 95 168 53
    87# 70 127 49
    93 124 137 49
Test Chemical Acetohexamide 
125         
 88 87 152 57 56
    93 93 128 46
    89 81 176 66
  
250         
 120r 77 212 59 56
    100# 99 130 43
    101 96 208 68
  
500         
 116 88 217 62 56
    103 93 155 50
    124r 101 183 49
  
750         
 114 91 259 76 69
    133r 119 227 57
    120 101 224 62
  
1000         
 138r 129 198 48
    121r 92 253 70
    126r 93 258 68
  
1500         
 134r 93 244 61
    37r 31 269 240
    131r 91 246 63
Positive Control 3-Methylcholanthrene 
2.5       
 85 26 894 351 310*
    102 57 777 253
    82 45 808 327

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 117 112 227 64 70
    90 88 272 101
    86 94 78 30
    112 105 279 83
Test Chemical Acetohexamide 
125         
 92# 59 111 40 50
    74 63 122 55
    85 57 143 56
  
250         
 69# 54 81 39 46
    74 53 90 40
    99 64 169 57
  
500         
 70 69 96 46 41
    103 48 112 36
    98 42 119 40
  
750         
 86 42 191 74 57
    72 48 70 33
    84 46 164 65
  
1000         
 108# 71 143 44 46
    118 61 170 48
    99 50 132 45
  
1500         
 159r 77 409 86
    110r 33 233 70
    149r 62 315 71
Positive Control 3-Methylcholanthrene 
2.5       
 53# 7 908 567 434*
    36# 19 378 352
    75# 36 867 384

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination