| Study Attribute | Value | 
|---|---|
| CEBS Accession Number: | 002-01606-0002-0000-7 | 
| Chemical Name: | Acrylonitrile | 
| CASRN: | 107-13-1 | 
| NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics | 
| NTP Study ID: | 269328_CA | 
| Study Result: | Positive | 
| Activation | Trial | Trial Call | 
|---|---|---|
| No Activation | 1 | Negative | 
| Induced Rat Liver S9 | 1_S9 | Weakly Positive | 
| No Activation | 2 | Negative | 
| Induced Rat Liver S9 | 2_S9 | Weakly Positive | 
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 
| Dimethyl Sulfoxide | 0 | 100 | 4 | 0.040 | 4.0 | 1 | 0.010 | 1.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | Acrylonitrile | 5 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 
| 10 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| 25 | 100 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | 3 | 0.030 | 2.0 | 0 | 0.000 | 0.0 | ||
| 50 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
| Positive Control: | Mitomycin-C | 0.5 | 100 | 99 | 0.990 | 63.0 | 26 | 0.260 | 25.0 | 43 | 0.430 | 32.0 | 30 | 0.300 | 28.0 | 
| Trend: | -0.385 | -0.385 | -0.519 | ||||||||||||
| Probability: | 0.650 | 0.650 | 0.698 | ||||||||||||
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 
| Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | Acrylonitrile | 1 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 
| 5 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| 10 | 100 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
| 25 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| 50 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
| 100 | 100 | 6 | 0.060 | 6.0 | 1 | 0.010 | 1.0 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | ||
| 200 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| Positive Control: | Cyclophosphamide | 50 | 100 | 74 | 0.740 | 51.0 | 16 | 0.160 | 15.0 | 40 | 0.400 | 32.0 | 18 | 0.180 | 17.0 | 
| Trend: | 3.206 | 1.613 | 2.794 | ||||||||||||
| Probability: | 0.001 | 0.053 | 0.003 | ||||||||||||
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 
| Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | Acrylonitrile | 5 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 
| 10 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| 25 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| 50 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
| 75 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| Positive Control: | Mitomycin-C | 0.25 | 100 | 53 | 0.530 | 38.0 | 18 | 0.180 | 17.0 | 25 | 0.250 | 21.0 | 10 | 0.100 | 10.0 | 
| Trend: | 0.224 | -0.391 | 1.417 | ||||||||||||
| Probability: | 0.411 | 0.652 | 0.078 | ||||||||||||
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 
| Dimethyl Sulfoxide | 0 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | Acrylonitrile | 25 | 100 | 4 | 0.040 | 4.0 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 
| 50 | 100 | 5 | 0.050 | 4.0 | 3 | 0.030 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
| 75 | 100 | 5 | 0.050 | 5.0 | 1 | 0.010 | 1.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
| 100 | 100 | 13 | 0.130 | 11.0 | 3 | 0.030 | 2.0 | 6 | 0.060 | 6.0 | 4 | 0.040 | 4.0 | ||
| 150 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| Positive Control: | Cyclophosphamide | 25 | 100 | 49 | 0.490 | 32.0 | 13 | 0.130 | 11.0 | 24 | 0.240 | 18.0 | 12 | 0.120 | 12.0 | 
| Trend: | 2.491 | -0.052 | 2.391 | ||||||||||||
| Probability: | 0.006 | 0.521 | 0.008 | ||||||||||||
 
                        