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Study Information
Study Attribute Value
CEBS Accession Number: 002-01606-0014-0000-0
Chemical Name: Acrylonitrile
CASRN: 107-13-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 189387
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 72 107 55 25 33
    69 92 91 44
    85 105 58 23
    77 96 88 38
Test Chemical Acrylonitrile 
3.13      
 67 53 64 32 31
    79 53 78 33
    62 69 53 28
  
6.25      
 70 63 74 35 37
    58 71 61 35
    52 68 65 41
  
12.5       
 86 71 138 53 71*
    71 47 186 88
  
25         
 79 31 346 147 212*
    72 31 337 156
    40 10 395 333
  
50         
 46 3 588 428 414*
    34 3 491 479
    50# 3 500 336
  
100         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 49 50 684 462 449*
    49 42 686 464
    54 50 682 421

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 97 120 83 29 34
    68# 83 90 44
    78 97 69 30
Test Chemical Acrylonitrile 
 0.005     
 45 51 35 26 24
    69 66 45 22
  
 0.01      
 64# 69 59 31 23
    52 61 27 17
    84 71 52 21
  
 0.02      
 55 57 69 42 49
    83 51 142 57
    64# 56 95 49
  
 0.03      
 74 41 164 74 72*
    99# 46 226 76
    78# 46 155 67
  
 0.04      
 68# 29 226 111 142*
    74 24 381 173
  
 0.05      
 53 7 365 230 192*
    88 22 407 155
Positive Control Methyl Methane Sulfonate 
10         
 26 6 447 562 562*
Methyl Methane Sulfonate 
250         
 59 37 820 465 465*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination