Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01606-0014-0000-0
Chemical Name:	Acrylonitrile
CASRN:	107-13-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	189387
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	72	107	55	25	33
			69	92	91	44
			85	105	58	23
			77	96	88	38
Test Chemical	Acrylonitrile	3.13	67	53	64	32	31
			79	53	78	33
			62	69	53	28
		6.25	70	63	74	35	37
			58	71	61	35
			52	68	65	41
		12.5	86	71	138	53	71*
			71	47	186	88	71*
		25	79	31	346	147	212*
			72	31	337	156
			40	10	395	333
		50	46	3	588	428	414*
			34	3	491	479
			50#	3	500	336
		100


Positive Control	Ethyl Methane Sulfonate	250	49	50	684	462	449*
			49	42	686	464
			54	50	682	421

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	97	120	83	29	34
			68#	83	90	44
			78	97	69	30
Test Chemical	Acrylonitrile	0.005	45	51	35	26	24
			69	66	45	22	24
		0.01	64#	69	59	31	23
			52	61	27	17
			84	71	52	21
		0.02	55	57	69	42	49
			83	51	142	57
			64#	56	95	49
		0.03	74	41	164	74	72*
			99#	46	226	76
			78#	46	155	67
		0.04	68#	29	226	111	142*
			74	24	381	173	142*
		0.05	53	7	365	230	192*
			88	22	407	155	192*
Positive Control	Methyl Methane Sulfonate	10	26	6	447	562	562*
Positive Control	Methyl Methane Sulfonate	250	59	37	820	465	465*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination