Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01617-0008-0000-5
Chemical Name:	Aldicarb
CASRN:	116-06-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	145873
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	107	89	29	35
			95	100	91	32
			112	99	134	40
			101	94	113	37
Test Chemical	Aldicarb	1311	71	56	187	88	87*
			87	62	224	86	87*
		1638	103	65	276	89	88*
			91	59	239	88	88*
		2048	100	40	297	99	110*
			86	40	310	121	110*
		2560	75	34	265	118	111*
			82	45	255	104	111*
		3200	81	28	327	134	119*
			96	30	297	103	119*
Positive Control	Ethyl Methane Sulfonate	500	35	17	802	775	676*
			46	30	852	613
			40	26	758	640

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	104	56	29	25
			58	97	41	24
			58	106	48	27
			57	94	31	18
Test Chemical	Aldicarb	1476	67	103	47	23	32
			73	124	88	40	32
		2952	62#	78	64	34	25
			72	72	36	17	25
		3281	78	71	68	29	31
			85	74	83	32	31
		3645	89	58	40	15	25
			78	53	80	34	25
		4050	74	32	68	31	27
			49	14	34	23	27
		4500	59	15	37	21	28
			40	8	43	36	28
		5000

Positive Control	Ethyl Methane Sulfonate	500	45	57	915	673	687*
			40	48	772	643
			38	41	838	745

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	97	72	34	35
			85	121	104	41
			73	110	71	32
			72	72	74	34
Test Chemical	Aldicarb	839	70	82	117	56	54*
			57	76	89	52
			65	87	107	55
		1049	67	78	100	50	48
			72	74	120	56
			53	74	58	37
		1311	75	81	142	63	58*
			68	65	107	53	58*
		1638	70	60	125	59	50
			65	55	74	38
			80	66	124	51
		2048	71	51	129	61	53*
			87	58	97	37
			71	53	129	61
		2560	70	35	144	69	74*
			68	33	168	82
			67	34	141	70
		3200	59	12	156	88	95*
			67	13	167	83
			60	10	203	114
		4000

		5000


Positive Control	Ethyl Methane Sulfonate	500	57	55	882	520	489*
Positive Control			56	51	765	457	489*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	104	185	60	58
			95	107	142	50
			88#	91	151	57
			90#	97	171	63
Test Chemical	Aldicarb	1049	93	99	196	71	66
			111	114	203	61	66
		1310	85#	87	192	75	66
			117#	120	199	56	66
		1638	105	103	227	72	86
			86	84	259	100	86
		2048	108	105	225	69	70
			108	100	229	71	70
		2560	91	81	249	91	90*
			81	72	213	88	90*
		3200	88#	76	267	101	102*
			91	81	282	104	102*
		4000	87	61	301	116	132*
			84	48	371	148	132*
Positive Control	3-Methylcholanthrene	5	73	59	673	306	289*
			80	65	662	275
			80	61	685	285

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	113#	113	150	44	42
			98	100	109	37
			89	90	102	38
			100	97	146	49
Test Chemical	Aldicarb	1048	116	75	240	69	60
			115	80	176	51	60
		1310	110	90	169	51	53
			116	83	193	55	53
		1638	111	80	240	72
		2048	109	82	150	46	48
			107	81	159	49	48
		2560	89	80	163	61	61
			109	83	196	60	61
		3200	103	84	144	46	69*
			94	76	254	91	69*
		4000	102	68	307	100	91*
			91	56	222	82	91*
Positive Control	3-Methylcholanthrene	5	55	33	633	381	354*
			76#	38	738	323
			71	32	764	359

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination