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Study Information
Study Attribute Value
CEBS Accession Number: 002-01617-0009-0000-6
Chemical Name: Aldicarb
CASRN: 116-06-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 566841
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
86 113 42 16 19
    77 100 48 21
    69 87 41 20
    75 101 41 18
Test Chemical Aldicarb
78         
84 104 54 21 17
    78 97 30 13
 
156         
79 107 43 18 21
    74 83 51 23
 
313         
88 105 50 19 20
    89 106 54 20
 
625         
95 87 59 21 17
    108# 151 46 14
 
1250         
96 81 74 26 20
    86 87 35 14
 
2500         
91 43 78 29 28
    94 59 78 28
 
5000         
   
Positive Control Ethyl Methane Sulfonate
500         
36 17 680 638 740*
    36 20 758 695
    30 14 797 886

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
83# 91 96 39 30
    77 95 76 33
    83 116 61 25
    94 98 70 25
Test Chemical Aldicarb
125         
65 71 67 34 29
    68 87 60 30
    85# 110 61 24
 
250         
76 102 57 25 23
    75 134 46 20
    88 120 62 24
 
500         
86 110 47 18 30
    86 72 96 37
    64 62 65 34
 
1000         
98 75 68 23 26
    107 140 78 24
    86 98 81 32
 
2000         
87 76 60 23 24
    93 80 68 24
    73 56 54 25
 
3000         
95 44 105 37 35
    155r 109 78 17
    100 74 99 33
Positive Control Ethyl Methane Sulfonate
500         
45 38 640 471 654*
    35 22 687 657
    25 10 618 835

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
81 119 135 56 59
    78# 87 166.5 71
    79 101 105 44
    68 94 136 67
Test Chemical Aldicarb
250         
75 75 131 58 64
    66 91 138 70
 
500         
54# 83 166 103 94*
    61 82 164 90
    61 72 161 88
 
1000         
70 89 156 74 94*
    63# 71 186 98
    73 84 242 110
 
2000         
66 64 283 143 123*
    54 67 203 126
    56# 58 170 102
 
3000         
52 42 204 131 122*
    61 51 259 143
    61 70 170 93
 
4000         
62# 11 351 189 170*
    59 8 315 178
    67 24 288 143
 
5000         
   
   
Positive Control 3-Methylcholanthrene
7         
48 28 332 231 198*
    58# 39 301.5 174
    60# 41 340.5 189

Non-Induced S9 Trial: 2 Experiment Call:
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
82 106 60 24 30
    91 84 83 30
    96 100 104 36
    92 111 78 28
Test Chemical Aldicarb
500         
97 68 132 46 37
    92 79 95 34
    97 95 94 32
 
1000         
94# 109 93 33 44*
    90 95 133 49
    86 86 130 51
 
2000         
101# 92 171 56 53*
    98 91 149 51
    93 90 145 52
 
3000         
90 99 121 45 60*
    91 81 200 73
    94 73 170 61
 
4000         
96# 72 184.5 64 66*
    97 67 187 64
    101 84 210 69
 
5000         
95 62 222 78 99*
    99# 6 324 110
    74 22 242 109
Positive Control 3-Methylcholanthrene
7         
67 66 302 150 149*
    86 68 346 135
    77 85 370 161

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
83 103 88 36 35
    81 114 80 33
    59 88 81 46
    65 95 54 28
Test Chemical Aldicarb
78         
72 105 83 38 37
    69 82 73 35
 
156         
88 89 87 33 37
    78 92 95 41
 
313         
81 80 91 37 30
    81 108 57 23
 
625         
84 88 75 30 29
    94 116 78 28
 
1250         
46 59 73 53 41
    50 75 42 28
 
2500         
64 73 120 63 60*
    68 76 116 57
 
5000         
   
Positive Control 3-Methylcholanthrene
5         
27 17 410 503 409*
    36 18 395 364
    33 16 360 360

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
90 103 72 27 28
    95 104 67 24
    89 98 83 31
    85 95 78 31
Test Chemical Aldicarb
500         
81# 97 55.5 23 24
    100 112 76 25
    101# 103 75 25
 
1000         
96 131 72 25 24
    73 96 55 25
    69 85 47 23
 
2000         
89 76 82 31 32
    99# 98 97.5 33
    102 95 98 32
 
3000         
87 69 95 36 35
    91 99 97 36
    85 78 86 34
 
4000         
95 54 149 52 43*
    95 86 96 34
    97 72 121 42
 
5000         
89 15 247 93 70*
    82 28 126 51
    89 25 177 66
Positive Control 3-Methylcholanthrene
5         
40 18 419 346 307*
    47 26 360 254
    41 16 392 321

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
123r 91 85 23 27
    118 118 119 34
    88 87 56 21
    80 95 61 25
Test Chemical Aldicarb
500         
63# 92 60 32 49*
    52 56 106 68
    74 83 106 48
 
1000         
75 77 100 44 60*
    91# 76 133.5 49
    66 35 170 86
 
2000         
65 99 94 49 52*
    64 53 150 78
    80 113 75 31
 
3000         
102 111 215 70 75*
    62 13 144 78
    80# 15 181.5 76
 
4000         
101 72 192 63
   
   
 
5000         
   
   
Positive Control 3-Methylcholanthrene
5         
13# 2 117 312 306*
    32 4 240 254
    15 1 159 353

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
109 91 68 21 22
    92 128 51 19
    96 97 60 21
    99 84 80 27
Test Chemical Aldicarb
500         
95 78 78 27 27
    92 78 74 27
    88 70 74 28
 
1000         
80 62 107 45 35*
    81 83 70 29
    78 85 75 32
 
2000         
93 80 84 30 34*
    97 64 95 33
    70 72 84 40
 
3000         
51 4 108 71 57*
    55 7 83 50
    72 20 108 50
 
4000         
   
   
Positive Control 3-Methylcholanthrene
5         
17 2 162 312 242*
    19 2 113 200
    17 2 109 214

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination