Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01633-0014-0000-0
Chemical Name:	Allyl isothiocyanate
CASRN:	57-06-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	352025
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	114	66	27	43
			71	109	70	33
			58	77	120	70
			45r	46	123	92
Test Chemical	Allyl isothiocyanate	0.05	72	87	79	36	55
			35	47	77	73	55
		0.1	80	81	127	53	61
			29	37	60	69	61
		0.2	77	60	100	43	39
			57	58	59	35	39
		0.4	46	22	58	42	70
			31	12	90	97	70
		0.8	24#	5	71	98	69
			31#	7	38	41	69
Positive Control	Other Positive Control	15	6r	4	136	735	163*
Positive Control			30	17	145	163	163*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	93	145	52	60
			110	127	168	51
			81	90	178	74
			75	90	142	63
Test Chemical	Allyl isothiocyanate	0.2	68	58	165	81	74
			87	61	174	67	74
		0.4	64	15	236	122	109*
			53	14	152	96	109*
		0.6	40	6	189	159	145*
			39	6	151	131	145*
		0.8	26	3	179	228	247*
			18	2	144	267	247*
		1

Positive Control	Methyl Methane Sulfonate	15	46	34	257	188	198*
Positive Control			47	31	291	208	198*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	102	104	35	40
			92	102	107	39
			82	110	111	45
			77	87	98	42
Test Chemical	Allyl isothiocyanate	0.6	47	11	89	64	68*
			46	11	102	73	68*
		0.8	42	5	73	59	69*
			38	7	90	79	69*
		1	41	4	85	70	87*
			40	4	123	103	87*
		1.2	34	4	99	97	94*
			32	3	87	90	94*
		1.4	20	2	136	227	196*
			23	2	113	165	196*
		1.6

Positive Control	Methyl Methane Sulfonate	15	42	43	184	145	124*
Positive Control			49	41	151	103	124*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination