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Study Information
Study Attribute Value
CEBS Accession Number: 002-01647-0001-0000-1
Chemical Name: Ampicillin trihydrate
CASRN: 7177-48-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 600069_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 04/16/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1045 425 0.407 8.50 27.0 2.859
Dimethyl Sulfoxide 
0         
 50 1042 412 0.395 8.24 26.5 0.000
Test Chemical Ampicillin trihydrate 
50         
 50 1044 446 0.427 8.92 26.5 8.045 
160         
 50 1048 465 0.444 9.30 26.5 12.218 
500         
 50 1042 473 0.454 9.46 26.5 14.806 
1500         
 50 1043 399 0.383 7.98 26.5 -3.248
Positive Control Mitomycin-C 
 0.001     
 50 1048 1198 1.143 23.96 27.0 189.112
Mitomycin-C 
 0.01      
 10 209 729 3.488 72.90 27.0 782.169
Trend: 0.041        
Probability: 0.484        

Trial #: 2    Activation: No Activation    Date: 06/07/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1039 389 0.374 7.78 26.0 0.000
Negative (Not Specified) 
0         
 50 1050 440 0.419 8.80 26.0 11.926
Test Chemical Ampicillin trihydrate 
500         
 50 1036 381 0.368 7.62 26.0 -1.773 
750         
 50 1044 374 0.358 7.48 26.0 -4.317 
1000         
 50 1046 423 0.404 8.46 26.0 8.013 
1500         
 50 1041 396 0.380 7.92 26.0 1.604
Positive Control Mitomycin-C 
 0.0E-4    
 50 1051 881 0.838 17.62 26.0 123.892
Mitomycin-C 
 0.01      
 10 209 662 3.167 66.20 26.0 746.014
Trend: 0.734        
Probability: 0.231        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/16/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 405 0.389 8.10 26.0 0.000
Negative (Not Specified) 
0         
 50 1043 433 0.415 8.66 26.0 6.811
Test Chemical Ampicillin trihydrate 
50         
 50 1043 390 0.374 7.80 26.0 -3.796 
160         
 50 1044 397 0.380 7.94 26.0 -2.163 
500         
 50 1046 439 0.420 8.78 26.0 7.981 
1500         
 50 1048 452 0.431 9.04 26.0 10.966
Positive Control Cyclophosphamide 
 0.3       
 50 1046 633 0.605 12.66 26.0 55.699
Cyclophosphamide 
2         
 10 207 415 2.005 41.50 26.0 415.811
Trend: 2.119        
Probability: 0.017        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 06/07/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 450 0.429 9.00 26.0 15.405
Dimethyl Sulfoxide 
0         
 50 1024 381 0.372 7.62 26.0 0.000
Test Chemical Ampicillin trihydrate 
500         
 50 1035 402 0.388 8.04 26.0 4.390 
750         
 50 1033 378 0.366 7.56 26.0 -1.652 
1000         
 50 1021 425 0.416 8.50 26.0 11.876 
1500         
 50 1043 340 0.326 6.80 26.0 -12.387
Positive Control Cyclophosphamide 
 0.3       
 50 1052 547 0.520 10.94 26.0 39.748
Cyclophosphamide 
2         
 10 210 334 1.590 33.40 26.0 327.467
Trend: -1.195        
Probability: 0.884