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Study Information
Study Attribute Value
CEBS Accession Number: 002-01647-0002-0000-2
Chemical Name: Ampicillin trihydrate
CASRN: 7177-48-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 600069_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 05/11/1984    Harvest Time: 11 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Ampicillin trihydrate 
50         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
1500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 30 0.300 25.0 12 0.120 12.0 17 0.170 16.0 1 0.010 1.0
Mitomycin-C 
1         
 50 42 0.840 56.0 22 0.440 32.0 19 0.380 28.0 1 0.020 1.0
Trend: -1.267 -0.498 -1.226  
Probability: 0.897 0.691 0.890  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 05/10/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 5 0.050 5.0 1 0.010 1.0 2 0.020 2.0 2 0.020 2.0
Test Chemical: Ampicillin trihydrate 
50         
 100 5 0.050 5.0 1 0.010 1.0 4 0.040 4.0 0 0.000 0.0 
160         
 100 4 0.040 4.0 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 
500         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
1500         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 44 0.440 34.0 29 0.290 26.0 15 0.150 14.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 44 0.880 58.0 19 0.380 30.0 24 0.480 40.0 1 0.020 1.0
Trend: -0.970 -1.059 0.192  
Probability: 0.834 0.855 0.424  


Trial #:2   Activation: No Activation    Date: 06/14/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Ampicillin trihydrate 
250         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
1000         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
1500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 18 0.180 16.0 11 0.110 10.0 7 0.070 7.0 0 0.000 0.0
Mitomycin-C 
1         
 50 25 0.500 40.0 21 0.420 36.0 4 0.080 8.0 0 0.000 0.0
Trend: 0.719 0.837 0.345  
Probability: 0.236 0.201 0.365  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 06/13/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Ampicillin trihydrate 
250         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 3 0.030 3.0 0 0.000 0.0 2 0.020 2.0 1 0.010 1.0 
1000         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1500         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 32 0.320 24.0 15 0.150 12.0 17 0.170 15.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 26 0.520 38.0 17 0.340 26.0 9 0.180 18.0 0 0.000 0.0
Trend: 0.534 -0.093 0.803  
Probability: 0.297 0.537 0.211