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Study Information
Study Attribute Value
CEBS Accession Number: 002-01647-0008-0000-8
Chemical Name: Ampicillin trihydrate
CASRN: 7177-48-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 351941
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 126 262 97 112
    97 102 283 98
    75# 71 318 141
Test Chemical Ampicillin trihydrate 
156         
 88# 82 336 127 128
    73 81 302 139
    55# 79 193.5 117
  
313         
 87 85 312 120 101
    89 108 289 108
    73# 88 163.5 75
  
625         
 90 91 279 103 96
    92 78 246 89
  
1250         
 74 88 264 119 112
    86 91 258 100
    76# 89 268.5 118
  
2500         
 94# 103 262.5 93 103
    74 88 235 106
    81 74 267 110
  
5000         
 64# 72 275 144 125
    100 85 312 104
    83 66 315 127
Positive Control Ethyl Methane Sulfonate 
250         
 38 18 878 780 648*
    59 27 997 562
    53 23 955 603

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89# 104 187 70 55
    101# 93 161 53
    88# 97 123 46
    112# 106 167 50
Test Chemical Ampicillin trihydrate 
156         
 93 100 172 61 63
    83 85 159 64
  
313         
 99 105 154 52 49
    108 116 130 40
    89# 81 143 54
  
625         
 99 108 133 45 48
    93 106 172 62
    105 125 120 38
  
1250         
 106 119 165 52 60
    99 92 210 71
    93 100 163 58
  
2500         
 94 98 180 64 64
    94 98 206 73
    112 128 184 55
  
5000         
 99 125 131 44 51
    94 91 166 59
    96 99 147 51
Positive Control Ethyl Methane Sulfonate 
250         
 93# 64 1104 396 350*
    107# 69 977 304

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 94 93 76 27 30
    75 108 59 26
    89 91 69 26
    80 107 94 39
Test Chemical Ampicillin trihydrate 
500         
 76 92 76 33 34
    73 110 88 40
    79 117 64 27
  
1000         
 66 128 46 23 21
    80# 120 45 19
    77 98 46 20
  
2000         
 91 116 79 29 24
    73 110 47 21
    60 117 41 23
  
3000         
 76 64 102 45 39
    79 114 68 29
    69 72 93 45
  
4000         
 76 83 62 27 31
    80 107 72 30
    67 71 73 37
  
5000         
 80 72 64 27 23
    68 70 44 22
    79 97 48 20
Positive Control Methyl Methane Sulfonate 
5         
 89 77 459 173 183*
    79 105 542 229
    105 81 461 146

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 116 99 40 38
    75 93 69 31
    76 98 93 41
    89 93 109 41
Test Chemical Ampicillin trihydrate 
500         
 89 156 135 50 48
    123r 109 195 53
    83 126 115 46
  
1000         
 117 141 154 44 47
    103 109 159 51
    117 159 162 46
  
2000         
 117# 120 208 59 57*
    100 139 163 55
    103 141 182 59
  
3000         
 120 135 204 57 53
    97 147 144 49
    141r 155 169 40
  
4000         
 98 107 127 43 46
    122r 147 125 34
    108 151 161 50
  
5000         
 102 130 159 52 58*
    81 111 193 79
    109 179 141 43
Positive Control 3-Methylcholanthrene 
3.5       
 93# 94 433 155 164*
    79 82 409 173
    75 98 372 165

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 105 74 27 24
    110 132 82 25
    62 63 40 22
Test Chemical Ampicillin trihydrate 
500         
 69 81 58 28 27
    84 72 60 24
    63 80 55 29
  
1000         
 52# 47 41 26 29
    69# 76 45 22
    58 76 68 39
  
2000         
 51 57 48 32
  
3000         
 71 96 47 22 28
    74 76 77 35
    82 91 70 29
  
4000         
 84 50 110 44 40*
    89 71 91 34
    92 81 114 42
  
5000         
 66# 76 37 19 21
    80 90 56 23
Positive Control 3-Methylcholanthrene 
2.5       
 91 28 603 220 210*
    89 50 615 230
    100 82 541 180

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 115 96 95 28 23
    92 86 65 24
    89 111 47 18
    92 107 67 24
Test Chemical Ampicillin trihydrate 
500         
 94 107 60 21 25
    91 99 59 22
    64 77 59 31
  
1000         
 103 111 39 13 25
    95 103 81 28
    92 91 94 34
  
2000         
 93 109 66 24 27
    104 117 92 29
    123r 105 90 24
  
3000         
 86 102 89 35 26
    108 96 58 18
    121r 143 95 26
  
4000         
 123r 131 68 19
    127r 124 104 27
    99 121 80 27
  
5000         
 103 114 42 14 25
    82 99 70 29
    80 84 78 32
Positive Control 3-Methylcholanthrene 
2.5       
 87 58 658 252 228*
    108 74 636 197
    89 51 624 234

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination