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Study Information
Study Attribute Value
CEBS Accession Number: 002-01653-0008-0000-5
Chemical Name: Anilazine
CASRN: 101-05-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G52321
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 113 69 22 25
    109 91 61 19
    73 96 72 33
Test Chemical Anilazine 
 0.5       
 92 98 39 14 16
    99 117 52 18
  
1         
 93 82 60 21 22
    75 80 52 23
  
2         
 104 89 66 21 22
    96 96 63 22
  
3         
 68 48 55 27 27
    116 64 94 27
  
4         
 62 35 106 57 49*
    59 48 72 41
  
6         
 92 11 337 122 99*
    86 24 193 75
Positive Control Ethyl Methane Sulfonate 
500         
 76 51 779 342 406*
    57 40 780 453
    61 39 775 422

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 99 45 23 22
    61 82 42 23
    56 103 32 19
    75 116 46 20
Test Chemical Anilazine 
 0.625     
 68 105 24 12 19
    48# 62 37.5 26
  
1.25      
 53 84 34 21 20
    60 86 34 19
  
2.5       
 50 70 37 25 24
    54 62 38 24
  
3.75      
 47 51 27 19 19
    46 54 27 19
  
5         
 54 28 83 52 95*
    36 6 151 139
  
7.5       
 48 6 178 124
   
  
10         
   
Positive Control Ethyl Methane Sulfonate 
500         
 38 51 558 487 483*
    60 64 700 387
    35 32 608 574

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 94 57 26 30
    74 92 73 33
    71 96 75 35
    76 119 62 27
Test Chemical Anilazine 
2         
 67 79 71 35 39
    67 67 87 43
  
4         
 99 93 92 31 37
    103 81 130 42
  
6         
 73 63 114 52 49*
    94 47 127 45
  
8         
 79 56 144 61 66*
    78 29 166 71
  
10         
 95 35 244 86 116*
    70 8 304 145
  
12         
 77 29 178 77 110*
    75 13 318 142
  
16         
   
Positive Control 3-Methylcholanthrene 
5         
 49 24 463 313 311*
    43 36 389 302
    47 31 452 318

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 98 87 44 41
    76 95 70 31
    65 111 86 44
    60 96 79 44
Test Chemical Anilazine 
 0.94      
 98 128 79 27 29
    99 150 92 31
  
1.88      
 77 106 63 27 25
    99 133 68 23
  
3.75      
 88 95 62 23 26
    83 100 69 28
  
7.5       
 72 69 73 34 34
    97 93 98 34
  
12.5       
 52 12 131 84
  
15         
 73 8 275 126 129*
    71 9 278 131
Positive Control 3-Methylcholanthrene 
5         
 56 29 578 343 426*
    46 26 658 477
    42 18 572 458

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination