Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01653-0009-0000-6
Chemical Name:	Anilazine
CASRN:	101-05-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G87759
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	48r	64	54	37	37
			51	89	69	45
			61	94	61	33
			70	117	66	31
Test Chemical	Anilazine	1.81	78	97	130	56	52
			64	82	91	48	52
		2.13	60	67	121	68	58
			75	85	109	49	58
		2.51	85	84	86	34	36
			69	73	80	39	36
		2.95	68	49	168	82	90*
			78	54	231	98	90*
		3.47	69	33	235	114	104*
			70	37	199	95	104*
		4.08	51	14	274	181	179*
			50	15	264	177	179*
		4.8	23	3	153	225	225*
			23	3	158	226	225*
Positive Control	Ethyl Methane Sulfonate	500	33	33	698	716	600*
			68	87	820	404
			37	38	750	679

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	106	149	69	45
			78	98	91	39
			77	96	77	33
			85	101	99	39
Test Chemical	Anilazine	1.8	71	73	158	74	79*
			59	58	149	84	79*
		2.1	75	76	163	72	80*
			74	67	195	88	80*
		2.5	68	36	303	148	133*
			59	30	208	118	133*
		2.9	58	21	330	190	187*
			56	15	309	184	187*
		3.5	28	5	242	286	322*
			31	4	337	359	322*
		4.1

Positive Control	Ethyl Methane Sulfonate	500	44	43	811	612	627*
			44	46	859	656
			54	52	986	614

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	94	321	131	117
			87	110	282	108
			83	106	273	110
			84#	91	305	121
Test Chemical	Anilazine	9.8	78	87	390	166	166
			87	93	429	165	166
		12.3	70	61	515	245	235*
			81#	82	549	225	235*
		15.4	73	66	529	243	244*
			84	101	614	245	244*
		19.2	76	49	690	301	318*
			67	38	668	335	318*
		24	59	23	673	380	415*
			52	19	706	450	415*
		30

Positive Control	3-Methylcholanthrene	5	64	63	656	342	384*
			60	55	720	400
			55	60	671	410

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68	86	170	83	76
			83	99	169	68
			81	113	191	78
			80	102	176	74
Test Chemical	Anilazine	11.3	69	74	287	139	136*
			72	78	289	133	136*
		13.3	67	69	297	149	142*
			71	79	288	135	142*
		15.7	71	76	369	174	169*
			70	68	346	165	169*
		18.4	71	59	424	200	195*
			76	62	428	189	195*
		21.7	62	41	373	201	218*
			64	40	454	236	218*
		25.5	58	25	408	233	223*
			60	22	380	213	223*
		30	36	3	394	370	357*
			36	4	375	344	357*
Positive Control	3-Methylcholanthrene	5	53	51	583	366	370*
			54	48	570	352
			46	40	541	392

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	108	335	128	146
			92	111	353	128
			89	110	374	141
			57	71	316	186
Test Chemical	Anilazine	11.3	75	74	512	229	223*
			81	73	529	218	223*
		13.3	77	66	497	214	227*
			72	57	514	239	227*
		15.7	70	42	688	327	282*
			73	54	520	238	282*
		18.4	67	35	650	323	315*
			66	28	612	308	315*
		21.7	46	9	463	337	319*
			76	41	683	302	319*
		25.5	73	25	786	357	392*
			57	17	730	427	392*
		30	39	4	586	499	452*
			19	6	231	405	452*
Positive Control	3-Methylcholanthrene	5	68	61	711	351	387*
			81	69	801	332
			68	74	970	478

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination