Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01654-0011-0000-0
Chemical Name:	Aniline
CASRN:	62-53-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	423002
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	57	104	42	24	24
			66	101	44	22
			53	95	38	24
			29r	45	21	25
Test Chemical	Aniline	3.3333	48	33	25	17	22
			45	102	37	28	22
		10	53	117	44	28	22
			50	91	25	17	22
		33.3333	56	102	57	34	31
			45	73	37	28	31
		100	52	78	36	23	23
			52	76	36	23	23
		333.3333	45	64	46	34	29
			43	66	32	25	29
		1000	36	46	41	38	36
			51	73	53	34	36
		3333.3333

Positive Control	Ethyl Methane Sulfonate	200	45	72	203	151	140*
Positive Control			39	68	150	129	140*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	71	89	68	32	29
			68	98	68	33
			77	111	41	18
			58	102	55	31
Test Chemical	Aniline	125	84	105	55	22	31
			72	101	85	39	31
		250	80	74	142	59	42
			71	79	53	25	42
		500	72#	65	79.5	37	40
			84#	70	109.5	43	40
		1000	79	63	92	39	40
			98	72	123	42	40
		2000	69	25	205	100	86*
			92	30	203	73	86*
Positive Control	Ethyl Methane Sulfonate	250	70#	91	312	149	148*
Positive Control			80#	84	352.5	147	148*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	64	96	134	70	69
			79	134	152	64
			64	116	133	69
			80	54	171	71
Test Chemical	Aniline	800	77	109	188	82	88
			60	76	166	92
			69	95	185	90
		1200	71	96	219	102	98
			65	93	194	100
			70	88	189	90
		1600	61	78	219	121	122*
			83	66	286	115
			88	76	341	129
		2000	84	49	396	157	161*
			69	54	364	175
			64	51	287	150
		2400	62	41	265	144	182*
			67	43	311	154
			59	18	434	247
Positive Control	Ethyl Methane Sulfonate	250	65	106	572	296	292*
Positive Control			82	113	710	289	292*

Nonactivation Trial: 4 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105	98	174	55	59
			98#	110	143	49
			84	92	183	73
Test Chemical	Aniline	1500	101	49	328	108	94*
			107	60	295	92
			92	58	223	81
		1750	105	47	277	88	94*
			101	50	241	80
			64	46	222	115
		2250	92	23	255	93	88*
			103	21	266	86
			94	19	238	85
		2500	105	15	241	76	83
			84	16	197	78
			104	15	297	96
Positive Control	Methyl Methane Sulfonate	15	31	22	210	225	220*
Positive Control			36	23	232	216	220*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	49r	100	76	52	42
			68#	104	84	41
			72	102	60	28
			55	94	93	56
Test Chemical	Aniline	62.5	62	99	81	43	45
			65#	89	90	46	45
		125	69#	99	121.5	59	55
			63#	81	97	52	55
		250	55	51	72	44	59
			53	77	117	73	59
		500	53	43	120	76	88*
			54	44	165	101	88*
		1000	55	21	163	99	94*
			57	14	152	89	94*
		2000

Positive Control	3-Methylcholanthrene	2.5	56	80	405	241	265*
Positive Control			43	72	373	288	265*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	97	98	249	86	81
			86	113	190	74
			104	108	261	84
			73	81	179	82
Test Chemical	Aniline	125	94	117	207	74	70
			102	128	206	67	70
		250	83	106	224	90	99
			81	113	262	108	99
		500	71	104	249	117	103
			66	113	177	89	103
		1000	65	64	249	128	144*
			68	75	324	159	144*
		2000	69	20	345	166	221*
			46	14	382	276	221*
Positive Control	3-Methylcholanthrene	2.5	34	17	860	839	736*
Positive Control			41	20	779	633	736*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	108	143	51	71
			81	114	178	74
			76#	78	132	58
			58	100	176	101
Test Chemical	Aniline	125	83	114	191	76	76
			90	108	206	76	76
		250	74	98	220	99	107
			86	71	296	115	107
		500	75	81	213	95	93
			77	89	210	91	93
		1000	62	58	213	115	114*
			74	87	251	114	114*
		2000	64	31	314	163	174*
			60	23	332	184	174*
Positive Control	3-Methylcholanthrene	2.5	58	21	731	423	454*
Positive Control			41	25	591	486	454*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination