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Study Information
Study Attribute Value
CEBS Accession Number: 002-01656-0004-0000-4
Chemical Name: Anthracene
CASRN: 120-12-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G25719
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 85 82 30 30
    107 115 97 30
Test Chemical Anthracene 
58.8       
 95 175 62 22 24
    87 150 67 26
  
84         
 89 165 87 32 29
    111 199 82 25
  
120         
 99 81 85 29 32
    109 131 114 35
  
172         
 99 87 140 47 43
    106 95 123 39
  
245         
 71 14 60 28 27
    118 60 93 26
  
350         
 121r 90 111 30
    97r 97 80 28
  
500         
 107 87 109 34 36
    100 57 115 38
Positive Control Ethyl Methane Sulfonate 
500         
 54 33 968 596 533*
    75 45 1059 471

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 104 158 56 63
    92 96 193 70
Test Chemical Anthracene 
246         
 76 80 113 50 46
    90 94 117 43
  
312         
 108 118 126 39 44
    100 109 145 48
  
394         
 150r 151 235 52
    87r 96 104 40
  
499         
 81 88 118 49 48
    93 89 131 47
  
632         
 89 85 132 49 51
    94 98 149 53
  
800         
 94 79 148 53 47
    104 95 127 41
Positive Control Ethyl Methane Sulfonate 
500         
 67 54 883 437 431*
    71 54 898 425

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 102 104 183 60 60
    103 96 185 60
Test Chemical Anthracene 
2.46      
 83 111 170 68 73
    87 78 203 78
  
4.92      
 90 79 247 91 89
    96 92 248 86
  
6.14      
 105 87 313 100 104*
    97 80 316 109
  
7.68      
 78 59 409 175 174*
    67 61 350 174
  
9.6       
 90 68 329 122 134*
    75 53 331 146
  
12         
 84 56 391 156 144*
    83 58 332 133
  
15         
 65 31 427 220 185*
    82 39 367 150
Positive Control 3-Methylcholanthrene 
5         
 34 15 444 433 409*
    43 17 499 385

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 99 353 137 133
    98 101 378 129
Test Chemical Anthracene 
2.46      
 68 76 197 97 129
    74 79 358 162
  
4.92      
 93 85 449 161 142
    87 82 323 124
  
6.14      
 64 49 439 229 201
    75 61 391 174
  
7.68      
 70 46 431 205 182
    95 55 449 158
  
9.6       
 73 32 428 196 200
    69 29 420 204
  
12         
 36 11 456 422 322*
    72 21 477 221
  
15         
 54 8 523 323 354*
    46 7 526 385
Positive Control 3-Methylcholanthrene 
5         
 32 11 453 477 511*
    31 11 498 544

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination