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Study Information
Study Attribute Value
CEBS Accession Number: 002-01684-0008-0000-9
Chemical Name: Barium chloride dihydrate
CASRN: 10326-27-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 741714
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 102 112 77 25 25
    87 94 58 22
    81 93 81 33
    92 101 58 21
Test Chemical Barium chloride dihydrate 
62.5       
 70 62 66 32 31
    79 84 85 36
    82 93 61 25
  
125         
 80 92 76 32 35
    89 89 82 31
    82 68 101 41
  
250         
 82 75 74 30 27
    92 118 79 29
    87 87 61 23
  
500         
 82 70 97 40 32
    100 88 76 25
    74 70 69 31
  
750         
 93 63 72 26 26
    100 73 76 25
    86 59 70 27
  
1000         
 84 49 83 33 26
    89 59 53 20
    90 35 64 24
Positive Control Methyl Methane Sulfonate 
5         
 52 30 499 317 288*
    74 49 479 215
    51 29 510 333

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 63 87 83 44 41
    145r 191 122 28
    121r 98 108 30
    96 113 111 39
Test Chemical Barium chloride dihydrate 
62.5       
 81# 94 98 40 43
    127r 88 64 17
    63 54 85 45
  
125         
 84 95 103 41 34
    80 63 87 36
    100 128 74 25
  
250         
 140r 160 91 22
    197r 139 114 19
    110 114 90 27
  
500         
 200r 186 81 13 22
    118 124 65 18
    98 114 76 26
  
750         
 114 108 103 30 27
    102 87 73 24
    142r 144 86 20
  
1000         
 99 61 76 26 34
    67# 34 84 42
    168r 67 101 20
Positive Control Methyl Methane Sulfonate 
5         
 61 62 504 277 214*
    95 100 478 167
    85 74 506 198

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 144r 157 108 25 25
    68# 79 55 27
    145r 158 60 14
    108 121 76 23
Test Chemical Barium chloride dihydrate 
31.3       
 156r 150 130 28 39
    95 128 140 49
    97# 109 82 28
  
62.5       
 77 39 79 34 50*
    61 38 97 53
    69 43 128 62
  
125         
 128r 39 191 50
    157r 51 141 30
    122r 38 131 36
  
250         
 94# 30 75 27 36
    60 19 93 51
    86 28 79 31
  
500         
 50 14 108 72 46*
    73 18 77 35
    68# 15 62 31
  
750         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 101# 124 788 260 405*
    31 46 506 550

Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 101 88 31 21
    82 96 56 23
    104 101 34 11
    107 103 59 18
Test Chemical Barium chloride dihydrate 
62.5       
 76 105 52 23 21
    83# 104 46 18
    7r 11 37 180
  
125         
 97 119 79 27 25
    101 108 71 23
    97 81 73 25
  
250         
 112 101 107 32 36*
    91 101 95 35
    60 65 75 41
  
500         
 94# 72 66 23 32*
    91 81 99 36
    107 87 118 37
  
750         
 84 55 95 38 37*
    92 78 101 37
    78 63 85 36
  
1000         
 96# 38 123 43 39*
    75 37 75 33
    87 28 106 41
Positive Control 3-Methylcholanthrene 
2.5       
 61 30 519 283 218*
    89 39 335 125
    69 29 508 245

Induced S9 Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 113 84 30 32
    86 102 71 28
    53 84 62 39
Test Chemical Barium chloride dihydrate 
62.5       
 52 68 35 22 32
    67 64 74 37
    45 56 50 37
  
125         
 86 69 55 21 33
    60 59 64 36
    63 34 82 44
  
250         
 57 49 60 35 29
    66 53 67 34
    64 52 33 17
  
500         
 86 27 125 49 49*
    58 17 91 52
    45 21 63 47
  
750         
 71 11 113 53 54*
    71 18 82 39
    59 11 124 70
  
1000         
 88 8 128 49 59*
    70 11 147 70
Positive Control 3-Methylcholanthrene 
2.5       
 86 68 637 246 279*
    95 59 656 231
    80 51 866 359

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination