Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01688-0010-0000-6
Chemical Name:	Benzaldehyde
CASRN:	100-52-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	895369
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	100	99	57	52
			81	95	122	51
			84	104	122	48
Test Chemical	Benzaldehyde	50	72	92	105	49	50
			60	101	92	51	50
		100	80	101	124	51	51
			90	103	136	51	51
		200	59	65	121	69	62
			80	94	130	54	62
		400	68	51	406	200	185*
			78	59	401	171	185*
		800

Positive Control	Methyl Methane Sulfonate	15	54	27	392	240	247*
Positive Control			45	32	339	254	247*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	87	111	62	53
			76	108	104	46
			70	110	111	53
			74	95	113	51
Test Chemical	Benzaldehyde	80	75	81	113	50	47
			91	92	140	52
			59	70	71	40
		160	66	78	99	50	57
			89	80	163	61
			80	74	142	59
		320	76	38	168	74	71
			92	40	161	58
			75	30	184	81
		480	50	10	168	112	98*
			82	13	203	82
			63	17	188	100
		640


Positive Control	Methyl Methane Sulfonate	15	21	15	187	297	314*
Positive Control			18	12	176	332	314*

Nonactivation Trial: 3 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	101	99	125	41	44
			95	103	122	43
			84	99	118	47
			123r	112	121	33
Test Chemical	Benzaldehyde	350	116	69	134	39	49
			140r	69	176	42
			90	66	158	59
		400	125r	53	151	40
			127r	52	139	37
			68	47	157	77
		450	122r	48	174	48	33
			97	54	97	34
			87	44	85	33
		500	53	29	114	72	55
			90	47	115	43
			81	48	123	51
Positive Control	Methyl Methane Sulfonate	15	35	21	389	372	348*
Positive Control			42	25	407	323	348*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination