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Study Information
Study Attribute Value
CEBS Accession Number: 002-01700-0002-0000-2
Chemical Name: Benzo(e)pyrene
CASRN: 192-97-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G17917
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 68 105 66 32 36
    65 82 76 39
Test Chemical Benzo(e)pyrene 
15.6       
 69 77 96 47 38
    103 132 93 30
  
31.25      
 62 82 93 50 49
    76 91 107 47
  
62.5       
 80 99 106 44 38
    76 95 71 31
  
125         
 89 96 71 27 32
    85 91 96 38
  
250         
 74 77 96 43 40
    76 86 84 37
Positive Control Ethyl Methane Sulfonate 
500         
 36 18 758 705 658*
    46 30 838 612
Negative Control Negative (Not Specified) 
 0         
 105 117 130 41 36*
    72 96 67 31

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 93 142 56 57
    89 102 154 57
Test Chemical Benzo(e)pyrene 
15.6       
 74 93 128 58 59
    68 81 123 60
  
31.25      
 97 97 165 57 59
    78 90 144 61
  
62.5       
 73 107 104 47 53
    73 79 126 58
  
125         
 82 82 102 42 51
    72 77 129 60
  
250         
 75r 92 92 41
Positive Control Ethyl Methane Sulfonate 
500         
 77 54 974 422 459*
    63 51 931 497
Negative Control Negative (Not Specified) 
 0         
 67 102 125 63 60*
    86 104 147 57

Non-Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 108 46 17 19
    90 86 59 22
Test Chemical Benzo(e)pyrene 
15         
 79 68 89 38 48*
    59# 37 105 59
  
30         
 66 34 77 39 44*
    51 35 75 49
  
60         
 78 74 94 40 41*
    61 48 76 41
  
80         
 62 55 78 42 43*
    53# 54 70.5 45
  
100         
 65 57 86 44 47*
    64 44 94 49
Positive Control 3-Methylcholanthrene 
7         
 80 49 297 124 128*
    83 57 330 132
Negative Control Negative (Not Specified) 
 0         
 85 100 63 25 27*
    66 106 56 28

Non-Induced S9 Trial: 2 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 92 187 86 93
    83 94 247 99
Test Chemical Benzo(e)pyrene 
31.3       
 73 82 205 93 96
    77 91 229 99
  
62.5       
 87# 89 297 114 113
    80 79 266 111
  
125         
 89 78 257 96 92
    87 83 229 88
  
187.5       
 87 72 256 99 98
    68 70 196 97
  
250         
 68 81 226 112 115
    84 66 299 119
Positive Control 3-Methylcholanthrene 
7         
 89 37 563 212 215*
    68 38 442 218
Negative Control Negative (Not Specified) 
 0         
 84 108 189 75 81*
    91 107 239 88

Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 55 78 152 92 103
    56 72 190 113
Test Chemical Benzo(e)pyrene 
 0.313     
 50 54 169 112 103
    56 63 159 94
  
 0.625     
 64 62 203 106 104
    73# 54 224 103
  
1.25      
 41 31 178 147 125
    52 40 161 103
  
2.5       
 71 26 362 171 163
    59 30 273 155
  
5         
    73 18 387 178
  
7.5       
   
Positive Control Dimethylnitrosoamine 
100         
 6r 4 44 238
    8r 5 52 224
Negative Control Negative (Not Specified) 
 0         
 68 133 158 77 77*
    70 117 160 76

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 134 192 87 102
    50 66 177 118
Test Chemical Benzo(e)pyrene 
 0.625     
 43 54 205 158 199*
    40 46 287 240
  
1.25      
 53 33 399 251 241*
    40 36 273 230
  
2.5       
 51 28 411 270 287*
    60 27 542 303
  
5         
 78 12 632 271 364*
    36 7 497 456
Positive Control 3-Methylcholanthrene 
7         
   
Negative Control Negative (Not Specified) 
 0         
 37r 83 153 140
    47r 67 139 100

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 62 85 55 30 28
    70 115 56 27
Test Chemical Benzo(e)pyrene 
 0.313     
 49 71 38 26 28
    41 73 38 31
  
 0.625     
 40# 60 35 29 38
    42 63 59 46
  
1.25      
 40 50 61 50 44
    54 54 60 37
  
2.5       
 65 26 105 54 57*
    62 38 111 60
  
5         
 78 7 228 97 85*
    54 7 117 72
  
7.5       
   
Positive Control 3-Methylcholanthrene 
5         
 23 2 421 606 674*
    16 2 364 743
Negative Control Negative (Not Specified) 
 0         
 38r 80 26 23
    50r 98 56 37

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 113 121 102 30
    123r 121 114 31
Test Chemical Benzo(e)pyrene 
1.25      
 112 56 216 64 74*
    76 45 190 84
  
2.5       
 93 40 306 109 103*
    83 52 240 96
  
5         
 95 37 294 103 139*
    78 28 410 175
  
7.5       
 92 51 462 167 163*
    94 71 449 159
  
10         
 89 59 422 159 138*
    120 48 423 118
Positive Control 3-Methylcholanthrene 
5         
 68 26 563 276 282*
    58 20 503 287
Negative Control Negative (Not Specified) 
 0         
 133r 121 132 33
    116 79 112 32

Induced S9 Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 83 151 64 48
    103 115 160 52
    68 90 97 47
    96 113 85 30
Test Chemical Benzo(e)pyrene 
1         
 72 58 325 151 133*
    81 67 277 114
    85 57 340 134
  
2         
 75 51 393 175 176*
    66# 35 376.5 191
    81 56 395 163
  
4         
 78 40 461 198 203*
    55 37 401 242
    81 42 411 169
  
5         
 60 29 459 257 201*
    73 28 413 189
    62# 23 291 156
  
6         
 62 28 389 210 183*
    85 32 471 184
    60# 5 279 156
  
8         
 75 19 382 169 212*
    55 12 417 254
Positive Control 3-Methylcholanthrene 
2.5       
 27 8 388 476 402*
    51 16 386 252
    28# 2 402 479

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination