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Study Information
Study Attribute Value
CEBS Accession Number: 002-01700-0003-0000-3
Chemical Name: Benzo(e)pyrene
CASRN: 192-97-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G65246
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 116 106 37 42
    79 84 112 47
Test Chemical Benzo(e)pyrene 
77.8       
 87 77 137 53 50
    79 76 112 47
  
130         
 83 75 140 57 56
    69 71 116 56
  
216         
 65 62 103 53 54
    69 64 113 55
  
360         
 91 77 150 55 53
    75 63 114 51
  
600         
 85 67 150 59 58
    84 68 142 56
  
1000         
 78 74 150 64 63
    83 67 155 62
Positive Control Ethyl Methane Sulfonate 
500         
 39 24 690 590 557*
    47 33 738 523

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 92 125 58 51
    76 108 100 44
Test Chemical Benzo(e)pyrene 
130         
 88 122 108 41 42
    93 123 118 42
  
216         
 86 105 185 72 70
    82 87 168 69
  
360         
 78# 95 120 51 42
    70 83 71 34
  
600         
 69 88 86 42 46
    72 92 106 49
  
1000         
 63 74 118 63 59
    55 67 90 55
Positive Control Ethyl Methane Sulfonate 
500         
 27 26 543 662 541*
    52# 49 661 420

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 102 100 110 36 40
    108 95 114 35
    93 101 134 48
    95 103 112 39
Test Chemical Benzo(e)pyrene 
32.8       
 92 85 108 39 41
    92 85 120 43
  
41         
 95 93 188 66 54
    107 105 158 49
    102 110 145 47
  
51.2       
 105 89 150 47 49
    97 100 135 46
    101 99 160 53
  
64         
 86 99 106 41 52
    86 88 145 57
    89 77 153 57
  
80         
 97 86 146 50 47
    112 100 143 43
    95 98 134 47
  
100         
 102 86 147 48 53
    97 106 113 39
    87 64 190 73
Positive Control Ethyl Methane Sulfonate 
500         
 60 38 1198 669 690*
    54 34 1246 764
    55 28 1053 636

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 112 106 128 38 35
    103 102 106 34
    88 92 102 39
    100 100 92 31
Test Chemical Benzo(e)pyrene 
21         
 89 99 119 44 42
    105 116 128 41
  
26.2       
 104 109 156 50 56*
    115 120 184 53
    99 98 188 64
  
32.8       
 101 106 117 39 39
    118 124 140 39
    108 84 130 40
  
41         
 104 119 150 48 42
    111 123 113 34
    108 113 138 43
  
51.2       
 95 74 146 51 44
    104 110 120 38
    90 89 110 41
  
64         
 103 98 101 33 41
    104 103 132 43
    97 72 140 48
  
80         
 121r 121 128 35
    123r 106 113 31
    113 88 110 33
Positive Control Ethyl Methane Sulfonate 
500         
 70 43 1230 583 580*
    74 47 1225 551
    72 25 1307 605

Non-Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 107 472 200 132
    76 93 147 65
Test Chemical Benzo(e)pyrene 
158         
 62 96 243 130 119
    69 107 225 108
  
211         
 54 57 330 205 164
    70 109 258 124
  
281         
 76 103 241 106 109
    72 98 241 112
  
375         
 70 96 240 114 121
    62 72 237 128
  
500         
 59 70 178 101 98
    68 77 196 96
Positive Control 3-Methylcholanthrene 
5         
 49 40 450 309 273*
    56 41 400 237

Non-Induced S9 Trial: 2 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 107 167 58 60
    72 93 134 62
Test Chemical Benzo(e)pyrene 
158         
 88 114 224 85 82
    85 76 200 78
  
211         
 91 97 236 86 86
    80 108 204 86
  
281         
 74 105 221 100 92
    83 114 210 85
  
375         
 80 113 188 78 83
    84 101 221 88
  
500         
 173r 222 157 30
    90r 111 163 60
Positive Control 3-Methylcholanthrene 
7         
 66 33 438 221 271*
    50 17 476 321
3-Methylcholanthrene 
10         
 5r 3 102 703
    8r 4 126 548

Non-Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 86 226 79 80
    100 111 216 72
    83 103 218 87
Test Chemical Benzo(e)pyrene 
51.66      
 103 75 201 65 66
    100 127 202 68
  
57.4       
 105 138 217 69 71
    98 115 215 73
  
63.8       
 104 112 185 59 70
    88 105 210 80
  
70.8       
 99 119 234 79 82
    93 112 237 85
  
87.5       
 60 63 203 112 116
    76 94 273 120
  
97.2       
 69 75 242 117 99
    97 103 235 81
  
108         
 88 92 269 102 96
    109 117 298 91
Positive Control 3-Methylcholanthrene 
7         
 64 38 411 214 245*
    52 31 424 272
    59 37 444 250

Non-Induced S9 Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 76 173 64 69
    84 111 194 77
    80 114 176 73
    88 99 166 63
Test Chemical Benzo(e)pyrene 
57.4       
 95 137 210 74 66
    88 101 155 59
  
63.8       
 83 108 168 67 76
    76 107 194 85
  
70.8       
 74 59 142 64 70
    75 68 171 76
  
78.7       
 91 117 177 65 73
    76 106 183 81
  
87.5       
 90r 104 187 69
Positive Control 3-Methylcholanthrene 
7         
 73 53 586 269 274*
    74 51 562 254
    60 57 540 300

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 86 132 46 48
    102 114 153 50
Test Chemical Benzo(e)pyrene 
5.8       
 90 83 144 54 62
    92 80 193 70
  
8.93      
 81 58 193 79 76
    101 62 224 74
  
13.7       
 89 34 193 72 61
    84 33 122 49
  
21.1       
 80 22 252 105 100*
    79 20 224 95
  
32.5       
 86 18 254 98
  
50         
 74 12 183 83 103*
    68 11 251 124
Positive Control 3-Methylcholanthrene 
5         
 75 65 414 184 197*
    69 59 434 211
3-Methylcholanthrene 
100         
 6r 3 103 624
    9r 5 129 487

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 92 30 11 12
    102 108 43 14
Test Chemical Benzo(e)pyrene 
3.77      
 101 104 67 22 18
    91 101 37 14
  
5.8       
 94 86 76 27 29*
    95 81 91 32
  
13.7       
 72 10 95 44 38*
    87 16 83 32
  
21.1       
 62 6 118 64
Positive Control 3-Methylcholanthrene 
6         
 63 28 270 143 214*
    41 19 347 286

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 99 104 125 42 50
    108 113 151 46
    80 85 130 54
    98 98 166 57
Test Chemical Benzo(e)pyrene 
3.8       
 95# 91 220 77 91*
    81 76 262 108
    94 90 249 88
  
5.8       
 98 90 250 85 100*
    79 76 268 113
    88 84 269 102
  
8.9       
 83 71 287 116 122*
    74 66 284 128
    75 67 273 121
  
13.7       
 83 62 332 134 127*
    87 57 320 122
    88 54 327 124
  
21.1       
 75 26 345 154 148*
    121r 34 409 113
    79 26 340 143
  
32.5       
 84 12 373 148 154*
    83 14 398 159
  
50         
 70 6 327 157 167*
    68 7 352 174
    67 5 344 172
Positive Control 3-Methylcholanthrene 
5         
 83 69 490 198 189*
    83 70 479 192
    77 65 413 178

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination