Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01705-0013-0000-9
Chemical Name:	Benzoin
CASRN:	119-53-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	542967
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	93	76	33	28
			66	100	47	24
			84	94	69	28
			73	113	59	27
Test Chemical	Benzoin	15.6	68	69	64	31	34
			74	63	74	33
			60	70	64	36
		31.3	82	78	74	30	34
			65	68	55	28
			71	47	92	43
		62.5	43	35	57	44	37
			57	44	51	30
			47	37	50	36
		125	76	16	125	55	46*
			69	14	89	43
			70	17	81	39
Positive Control	Ethyl Methane Sulfonate	250	43	30	704	552	588*
			37	30	727	655
			42	30	695	558

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	47r	43	51	37	21
			52#	75	24	15
			74	113	59	27
			66	112	39	20
Test Chemical	Benzoin	25	42#	63	49	39	39*
			54	84	65	40	39*
		50	56	74	33	20	27
			60	50	25	14
			49	57	68	46
		100	47	28	43	30	36*
			33	25	57	57
			50	44	29	19
		125	62	23	54	29	29
			51	39	38	25
			52	27	52	33
		150	57	15	53	31	26
			74	19	49	22
			78#	14	57	24
		200	68	11	83	40	34*
			66	5	62	31
			58	9	51	29
Positive Control	Ethyl Methane Sulfonate	250	54	57	445	273	289*
Positive Control			54	51	489	305	289*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	114	69	28	29
			98#	100	74	25
			86	87	86	33
Test Chemical	Benzoin	1.25	88#	81	59	22	24
			87	117	67	26	24
		2.5	79	101	71	30	29
			107	115	88	28	29
		5	92#	65	120	43	41
			97	61	105	36
			89	57	116	43
		10	76	36	120	53	61*
			91	28	192	70	61*
		15	65	23	163	84	83*
			73#	18	177	81	83*
		20	77	9	228	98	106*
			72#	8	276	127
			84#	15	229	91
		30


Positive Control	3-Methylcholanthrene	2.5	76#	61	515	226	213*
Positive Control			90#	58	539	201	213*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99#	125	80	27	27
			75#	92	47	21
			88	83	84	32
Test Chemical	Benzoin	1.25	78	85	70	30	25
			68#	75	46	23
			80#	94	56	23
		2.5	96#	91	61	21	24
			78	89	62	27	24
		5	64	44	102	53	53*
			72#	39	114	53	53*
		10	60#	16	200	112	101*
			45	16	138	101
			63#	17	170	89
		15	60	9	203	112

		20


Positive Control	3-Methylcholanthrene	2.5	77#	71	505	220	220*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination