Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01706-0002-0000-8 |
Chemical Name: | Benzonitrile |
CASRN: | 100-47-0 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 562549_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Negative |
Induced Rat Liver S9 | 1_S9 | Weakly Positive |
No Activation | 2 | Test Failure |
Induced Rat Liver S9 | 2_S9 | Weakly Positive* |
Induced Rat Liver S9 | 3_S9 | Test Failure |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 200 | 3 | 0.015 | 1.5 | 2 | 0.010 | 1.0 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 200 | 5 | 0.025 | 2.5 | 2 | 0.010 | 1.0 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 | |
Test Chemical: | Benzonitrile | 251.3 | 200 | 5 | 0.025 | 2.5 | 3 | 0.015 | 1.5 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
375 | 200 | 2 | 0.010 | 1.0 | 1 | 0.005 | 0.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | ||
750 | 200 | 4 | 0.020 | 2.0 | 3 | 0.015 | 1.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | ||
1500 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.25 | 200 | 32 | 0.160 | 12.5 | 18 | 0.090 | 8.0 | 14 | 0.070 | 6.5 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.75 | 25 | 11 | 0.440 | 32.0 | 4 | 0.160 | 16.0 | 7 | 0.280 | 24.0 | 0 | 0.000 | 0.0 | |
Trend: | -0.574 | 0.256 | -1.153 | ||||||||||||
Probability: | 0.717 | 0.399 | 0.875 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | Benzonitrile | 1005 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
1256 | 100 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
1495 | 100 | 13 | 0.130 | 11.0 | 3 | 0.030 | 3.0 | 10 | 0.100 | 9.0 | 0 | 0.000 | 0.0 | ||
1993 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 7.5 | 100 | 13 | 0.130 | 8.0 | 5 | 0.050 | 5.0 | 8 | 0.080 | 6.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 37.5 | 25 | 9 | 0.360 | 24.0 | 5 | 0.200 | 16.0 | 4 | 0.160 | 16.0 | 0 | 0.000 | 0.0 | |
Trend: | 2.553 | 0.657 | 2.843 | ||||||||||||
Probability: | 0.005 | 0.256 | 0.002 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 4 | 0.020 | 2.0 | 3 | 0.015 | 1.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Test Chemical: | Benzonitrile | 200 | 200 | 4 | 0.020 | 2.0 | 3 | 0.015 | 1.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Positive Control: | Mitomycin-C | 0.75 | 25 | 16 | 0.640 | 32.0 | 5 | 0.200 | 16.0 | 11 | 0.440 | 32.0 | 0 | 0.000 | 0.0 |
Trend: | 0.000 | 0.000 | 0.000 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 200 | 5 | 0.025 | 2.5 | 4 | 0.020 | 2.0 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 200 | 6 | 0.030 | 3.0 | 3 | 0.015 | 1.5 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 | |
Test Chemical: | Benzonitrile | 375 | 200 | 4 | 0.020 | 2.0 | 2 | 0.010 | 1.0 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
750 | 200 | 7 | 0.035 | 3.5 | 4 | 0.020 | 2.0 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 | ||
1500 | 25 | 23 | 0.920 | 72.0 | 3 | 0.120 | 8.0 | 20 | 0.800 | 64.0 | 0 | 0.000 | 0.0 | ||
3000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 7.5 | 200 | 26 | 0.130 | 10.0 | 7 | 0.035 | 3.5 | 19 | 0.095 | 8.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 37.5 | 25 | 11 | 0.440 | 32.0 | 6 | 0.240 | 24.0 | 5 | 0.200 | 20.0 | 0 | 0.000 | 0.0 | |
Trend: | 6.679 | 1.407 | 7.029 | ||||||||||||
Probability: | 0.000 | 0.080 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 9 | 0.045 | 4.5 | 4 | 0.020 | 2.0 | 5 | 0.025 | 2.5 | 0 | 0.000 | 0.0 |
Test Chemical: | Benzonitrile | 200 | 200 | 6 | 0.030 | 2.5 | 0 | 0.000 | 0.0 | 6 | 0.030 | 2.5 | 0 | 0.000 | 0.0 |
Positive Control: | Cyclophosphamide | 37.5 | 25 | 16 | 0.640 | 36.0 | 6 | 0.240 | 24.0 | 10 | 0.400 | 24.0 | 0 | 0.000 | 0.0 |
Trend: | 0.000 | 0.000 | 0.000 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |