Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01714-0009-0000-4
Chemical Name:	Benzyl alcohol
CASRN:	100-51-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	464779
Study Result:	Equivocal

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	110	88	46	50
			42r	92	55	44
			52	74	108	69
			70	116	76	36
Test Chemical	Benzyl alcohol	156.25	85	126	89	35	34
			92	95	94	34	34
		312.5	69	98	123	59	58
			71	112	119	56	58
		625	104	132	164	53	58
			88	86	166	63	58
		1250	95	134	134	47	57
			73	79	146	67	57
		2500	82	82	170	69	67
			94	78	181	65	67
Positive Control	Methyl Methane Sulfonate	15	28	27	213	254	222*
Positive Control			26	24	150	191	222*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	101	74	30	38
			86	101	121	47
			78	101	79	34
			63	97	77	41
Test Chemical	Benzyl alcohol	2500	82	97	92	38	37
			87	95	97	37	37
		3000	67	56	98	49	43
			81	70	91	37	43
		3500	75	56	104	46	43
			92	73	111	40	43
		4000	36	20	52	48	58
			63	41	128	68	58
		4500	76	21	263	115	106*
			104	20	302	97	106*
		5000

Positive Control	Methyl Methane Sulfonate	15	31	31	153	164	203*
Positive Control			27	29	194	242	203*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	62	90	101	54	48
			71#	99	115	54
			99	103	139	47
			102	108	111	36
Test Chemical	Benzyl alcohol	250	88	86	90	34	40
			60	73	83	46	40
		500	79	89	91	39	40
			88	86	110	42	40
		1500	67	68	75	37	33
			60	55	51	28	33
		2500	82	39	76	31	35
			85	53	100	39	35
		3500

Positive Control	Methyl Methane Sulfonate	15	40	24	206	172	227*
Positive Control			33	21	282	282	227*

Nonactivation Trial: 4 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	119	66	30	35
			56	110	76	45
			62	101	69	37
			61	70	52	28
Test Chemical	Benzyl alcohol	3200	67	73	76	38	27
			65	65	33	17	27
		3400	81	59	56	23	29
			81	56	85	35	29
		3600	71	43	57	27	27
			73	65	61	28	27
		3800	66	38	50	25	28
			81	32	73	30	28
Positive Control	Methyl Methane Sulfonate	15	31	32	137	147	135*
Positive Control			26	26	97	123	135*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	112	81	34	37
			66	87	91	46
			79	107	60	25
			60	95	81	45
Test Chemical	Benzyl alcohol	250	89	89	121	46	37
			81	112	67	28	37
		500	94	103	131	47	51
			82	93	134	55	51
		1500	75	96	111	49	46
			77	73	97	42	46
		2500	55	54	55	34	43
			84	64	134	53	43
		3500	79	13	185	78

		4500

Positive Control	3-Methylcholanthrene	2.5	58	35	490	279	279*
Positive Control			129r	83	634	164	279*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	104	59	29	29
			65	103	47	24
			62	87	68	37
			63	106	50	27
Test Chemical	Benzyl alcohol	250	66	106	72	36	37
			70	107	78	37	37
		500	51	79	61	40	33
			60	89	46	26	33
		1500	70	83	71	34	36
			60	83	69	39	36
		2500	75	76	60	27	23
			57	62	34	20	23
		3500

Positive Control	3-Methylcholanthrene	2.5	57	56	262	154	164*
Positive Control			63	59	327	173	164*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination