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Study Information
Study Attribute Value
CEBS Accession Number: 002-01715-0002-0000-8
Chemical Name: Benzyl chloride
CASRN: 100-44-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 186977_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #:1   Activation: No Activation    Date: 03/05/1986    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.0 0 0.000 0.0 3 0.015 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: Benzyl chloride 
1         
 200 3 0.015 1.0 0 0.000 0.0 3 0.015 1.0 0 0.000 0.0 
2.5       
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
5         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
7.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 26 0.130 12.5 11 0.055 5.5 15 0.075 7.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 9 0.360 32.0 2 0.080 8.0 7 0.280 24.0 0 0.000 0.0
Trend: -0.093 -1.307 0.366  
Probability: 0.537 0.904 0.357  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/04/1986    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: Benzyl chloride 
15         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
20         
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0 
49.5       
 200 10 0.050 4.0 2 0.010 1.0 8 0.040 3.0 0 0.000 0.0 
100         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 20 0.100 9.0 4 0.020 2.0 16 0.080 7.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 9 0.360 32.0 6 0.240 24.0 3 0.120 12.0 0 0.000 0.0
Trend: 2.432 1.612 1.865  
Probability: 0.008 0.053 0.031  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/13/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: Benzyl chloride 
20         
 200 8 0.040 4.0 2 0.010 1.0 6 0.030 3.0 0 0.000 0.0 
35         
 200 27 0.135 7.5 8 0.040 3.0 19 0.095 5.5 0 0.000 0.0 
50         
 100 49 0.490 23.0 20 0.200 14.0 29 0.290 16.0 0 0.000 0.0 
65         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 200 20 0.100 9.5 7 0.035 3.5 13 0.065 6.5 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 8 0.320 28.0 2 0.080 4.0 6 0.240 24.0 0 0.000 0.0
Trend: 6.389 5.263 5.360  
Probability: 0.000 0.000 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 03/27/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0
Test Chemical: Benzyl chloride 
30         
 200 22 0.110 8.0 9 0.045 4.5 13 0.065 4.5 0 0.000 0.0 
40         
 25 22 0.880 44.0 17 0.680 36.0 5 0.200 16.0 0 0.000 0.0 
50         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 200 34 0.170 16.5 10 0.050 5.0 24 0.120 11.5 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 12 0.480 40.0 6 0.240 24.0 6 0.240 24.0 0 0.000 0.0
Trend: 6.317 6.390 3.289  
Probability: 0.000 0.000 0.001