Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01715-0010-0000-7
Chemical Name:	Benzyl chloride
CASRN:	100-44-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	057128
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	96	215	74	73
			87	83	206	79
			107	95	193	60
			89	126	208	78
Test Chemical	Benzyl chloride	1.25	110	95	202	61	70
			93	94	218	78	70
		2.5	91	84	236	87	88
			79	94	209	89	88
		5	86	68	241	93	91
			68	76	178	88	91
		10	90#	66	245	91	102
			78	63	266	114	102
		20	78	45	508	217	231*
			75	43	550	246	231*
Positive Control	Methyl Methane Sulfonate	15	35	22	416	398	401*
Positive Control			41	20	497	404	401*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	95	92	53	58
			74	99	146	66
			68#	119	88.5	44
			65	88	135	70
Test Chemical	Benzyl chloride	2	74	84	126	57	58
			69	90	121	58	58
		4	63	95	131	69	69
			66	102	134	68	69
		8	104	117	216	69	93*
			62#	68	217.5	117	93*
		16	53	28	523	331	335*
			53	27	541	339	335*
		24	13	2	246	656	635*
			15	2	273	613	635*
		32

Positive Control	Methyl Methane Sulfonate	15	22	22	146	225	228*
Positive Control			20	17	137	232	228*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination