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Study Information
Study Attribute Value
CEBS Accession Number: 002-01715-0010-0000-7
Chemical Name: Benzyl chloride
CASRN: 100-44-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 057128
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 97 96 215 74 73
    87 83 206 79
    107 95 193 60
    89 126 208 78
Test Chemical Benzyl chloride 
1.25      
 110 95 202 61 70
    93 94 218 78
  
2.5       
 91 84 236 87 88
    79 94 209 89
  
5         
 86 68 241 93 91
    68 76 178 88
  
10         
 90# 66 245 91 102
    78 63 266 114
  
20         
 78 45 508 217 231*
    75 43 550 246
Positive Control Methyl Methane Sulfonate 
15         
 35 22 416 398 401*
    41 20 497 404

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 58 95 92 53 58
    74 99 146 66
    68# 119 88.5 44
    65 88 135 70
Test Chemical Benzyl chloride 
2         
 74 84 126 57 58
    69 90 121 58
  
4         
 63 95 131 69 69
    66 102 134 68
  
8         
 104 117 216 69 93*
    62# 68 217.5 117
  
16         
 53 28 523 331 335*
    53 27 541 339
  
24         
 13 2 246 656 635*
    15 2 273 613
  
32         
   
Positive Control Methyl Methane Sulfonate 
15         
 22 22 146 225 228*
    20 17 137 232

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination