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Study Information
Study Attribute Value
CEBS Accession Number: 002-01728-0014-0000-5
Chemical Name: Bisphenol A
CASRN: 80-05-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 831513
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 68 96 68 34 32
    87 111 79 30
    85 61 76 30
    74 131 78 35
Test Chemical Bisphenol A 
6.25      
 87 78 52 20 22
    93 76 62 22
    101 110 76 25
  
12.5       
 73 29 61 28 24
    93 98 55 20
    83 99 58 23
  
25         
 92 86 88 32 29
    79 51 60 25
    65 22 60 31
  
50         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 32 6 355 374 444*
    27 6 382 472
    26 7 375 487

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 120 104 105 29 30
    116 101 122 35
    106 99 89 28
    96 96 83 29
Test Chemical Bisphenol A 
5         
 113 97 113 33 35
    110 90 111 34
    111 77 123 37
  
10         
 113 82 87 26 25
    103 93 80 26
    120 109 86 24
  
20         
 116 76 82 23 25
    104 75 74 24
    85 83 68 27
  
30         
 101 65 90 30 28
    116 63 90 26
    112 72 92 27
  
40         
 107 20 89 28 28
    102 39 78 25
    90 26 80 30
  
50         
    85 13 69 27
   
  
60         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 142r 98 875 205 152*
    87 78 375 144
    67 67 323 160

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 99 88 69 23 27
    89 94 98 37
    95 111 82 29
    97 107 56 19
Test Chemical Bisphenol A 
5         
 86 88 124 48 36
    118 102 104 29
    88 81 79 30
  
10         
 110 102 120 36 40
    92 75 133 48
    94 89 97 34
  
20         
 88 56 97 37 38
    122r 82 100 27
    106 67 127 40
  
30         
 118 65 120 34 38
    115 83 120 35
    100 65 136 45
  
40         
 113 69 103 30 32
    117 62 113 32
    106 59 110 35
  
50         
 89 33 105 39 48*
    84 40 88 35
    61 16 129 70
  
60         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 49 35 369 249 279*
    63 51 521 276
    67 51 624 311

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 97 102 107 37 38
    90 110 81 30
    76 93 101 44
    81 95 97 40
Test Chemical Bisphenol A 
25         
 153r 81 119 26 59
    74 33 106 48
    75 38 158 70
  
50         
 84 28 95 38 32
    139r 43 123 30
    76 35 58 26
  
100         
 60 18 107 59 46
    48 14 61 42
    82 24 91 37
  
200         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 47 44 350 251 124*
    88# 101 74 28
    29 28 81 92

Induced S9 Trial: 2 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 79 117 166 70 74
    68 100 168 83
    72 110 154 72
    72 73 154 71
Test Chemical Bisphenol A 
5         
 53 86 104 66 63
    59 120 104 59
    61 119 119 66
  
10         
 65 119 121 62 51
    58# 119 87 50
    82 150 96 39
  
20         
 84 137 118 47 60
    85 146 122 48
    59 70 149 84
  
30         
 67 103 117 58 51
    88 97 137 52
    97 146 126 43
  
40         
 75 103 137 61
  
50         
 94 70 101 36 46
    83# 131 120 48
    85 135 134 53
Positive Control 3-Methylcholanthrene 
2.5       
 110 164 368 112 109
    110 191 349 106

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 104 106 156 50 46
    111 108 141 43
    114 128 149 44
    105 58 156 49
Test Chemical Bisphenol A 
5         
 121r 121 129 36 36
    116 128 108 31
    109 115 132 40
  
10         
 114 112 131 38 42
    111 121 136 41
    107 68 146 46
  
20         
 112 99 104 31 42
    85 62 135 53
    111 71 143 43
  
30         
 104 16 76 24 34
    100 34 128 43
    119 68 121 34
  
40         
 84 9 112 45 44
    107 13 133 42
    103 20 143 46
  
50         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 71 32 1065 499 476*
    48 12 769 534
    93 48 1103 396

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination