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Study Information
Study Attribute Value
CEBS Accession Number: 002-01736-0002-0000-1
Chemical Name: Boric acid
CASRN: 10043-35-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 153962_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 
Induced Rat Liver S9  3_S9  Negative 

Trial #:1   Activation: No Activation    Date: 02/21/1984    Harvest Time: 11.8 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Boric acid 
500         
 100 2 0.020 1.0 0 0.000 0.0 2 0.020 1.0 0 0.000 0.0 
1000         
 100 6 0.060 5.0 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 
1500         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
2000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 56 0.560 38.0 22 0.220 20.0 24 0.240 22.0 10 0.100 1.0
Mitomycin-C 
1         
 50 47 0.940 60.0 28 0.560 44.0 19 0.380 32.0 0 0.000 0.0
Trend: 0.329 0.205 0.326  
Probability: 0.371 0.419 0.372  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/05/1984    Harvest Time: 12.3 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Boric acid 
1000         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
1600         
 100 4 0.040 3.0 0 0.000 0.0 4 0.040 3.0 0 0.000 0.0 
2000         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
2500         
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 24 0.240 22.0 10 0.100 10.0 14 0.140 14.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 59 1.180 72.0 33 0.660 54.0 25 0.500 42.0 1 0.020 1.0
Trend: 1.892 0.754 1.860  
Probability: 0.029 0.226 0.031  


Trial #:2   Activation: No Activation    Date: 01/27/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Boric acid 
50         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
160         
 100 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0 1 0.010 1.0 
500         
 100 2 0.020 2.0 0 0.000 0.0 1 0.010 1.0 1 0.010 1.0 
1600         
 100 3 0.030 2.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.1       
 100 51 0.510 41.0 23 0.230 22.0 28 0.280 24.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 100 35 0.350 26.0 21 0.210 15.0 14 0.140 13.0 0 0.000 0.0
Trend: 0.217 0.002 0.581  
Probability: 0.414 0.499 0.281  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/26/1984    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Boric acid 
50         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
160         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
1600         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 7 0.070 7.0 3 0.030 3.0 4 0.040 4.0 0 0.000 0.0
Cyclophosphamide 
50         
 100 67 0.670 42.0 28 0.280 22.0 39 0.390 28.0 0 0.000 0.0
Trend: 0.817 1.417 -0.003  
Probability: 0.207 0.078 0.501  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 02/29/1984    Harvest Time: 15 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 1 0.010 1.0
Test Chemical: Boric acid 
1000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
1600         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2000         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
2500         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 72 0.720 39.0 39 0.390 25.0 23 0.230 20.0 10 0.100 1.0
Trend: 1.045 0.416 1.592  
Probability: 0.148 0.339 0.056