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Study Information
Study Attribute Value
CEBS Accession Number: 002-01736-0012-0000-2
Chemical Name: Boric acid
CASRN: 10043-35-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 621739
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 110 119 63 58
    69 102 98 48
    64 115 110 57
    67# 73 130.5 65
Test Chemical Boric acid 
62.5       
 64 81 124 65 67
    71 75 148 69
  
125         
 50 92 123 83 69
    55 113 93 56
  
250         
 69 61 170 83 72
    52 57 96 62
  
500         
 55 80 135 82 67
    71# 107 110 52
  
1000         
 60 69 131 73 66
    60 71 105 59
Positive Control Methyl Methane Sulfonate 
15         
 26 29 344 438 435*
    37 28 484 432

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 76 86 72 32 34
    60 108 70 39
    89 123 83 31
    79 83 83 35
Test Chemical Boric acid 
1000         
 70 109 79 38 38
    72 105 83 39
  
1800         
 75 92 81 36 36
    89# 94 97 36
  
2600         
 71 87 90 42 41
    73 103 90 41
  
3400         
 88# 90 130.5 49 43
    91 82 100 37
  
4200         
 99 86 146 49 48
    107 84 150 47
  
5000         
 95 79 160 56 50
    103 89 135 44
Positive Control Methyl Methane Sulfonate 
15         
 21 18 257 402 385*
    21 17 226 367

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 95 109 51 18 24
    89 126 84 31
    92 85 67 24
    78 79 54 23
Test Chemical Boric acid 
1800         
 100 77 66 22 21
    86 92 52 20
  
2600         
 115 78 64 19 22
    113 90 86 25
  
3400         
 78 81 44 19 25
    85 83 78 31
  
4200         
 87 64 68 26 24
    93 80 62 22
  
5000         
 104 62 62 20 22
    112 55 83 25
Positive Control Methyl Methane Sulfonate 
15         
 54 31 228 140 166*
    42 27 244 192

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 51 76 141 92 67
    94 109 136 48
    78 107 121 52
    81 108 185 76
Test Chemical Boric acid 
2000         
 62 92 107 57 51
    70 117 95 46
  
3000         
 87 115 156 60 85
    56 43 184 110
  
4000         
 98 116 155 53 52
    83 110 128 52
  
5000         
 89 103 140 52 53
    74 90 120 54
Positive Control 3-Methylcholanthrene 
2.5       
 66 72 340 171 182*
    62 67 358 193

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 70 87 175 84 90
    87# 103 223.5 85
    96 104 285 99
    80 106 222 92
Test Chemical Boric acid 
1000         
 83# 99 211.5 85 83
    73 90 176 81
  
2000         
 94# 90 278 99 98
    80 88 235 98
  
3000         
 79 71 263 111 109
    80 100 257 107
  
4000         
 73# 87 217.5 99 95
    100 77 273 91
  
5000         
 64# 57 175.5 92 114
    57 65 230 136
Positive Control 3-Methylcholanthrene 
2.5       
 59 34 867 488 536*
    45# 38 787 583

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination