Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01736-0012-0000-2
Chemical Name:	Boric acid
CASRN:	10043-35-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	621739
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	110	119	63	58
			69	102	98	48
			64	115	110	57
			67#	73	130.5	65
Test Chemical	Boric acid	62.5	64	81	124	65	67
			71	75	148	69	67
		125	50	92	123	83	69
			55	113	93	56	69
		250	69	61	170	83	72
			52	57	96	62	72
		500	55	80	135	82	67
			71#	107	110	52	67
		1000	60	69	131	73	66
			60	71	105	59	66
Positive Control	Methyl Methane Sulfonate	15	26	29	344	438	435*
Positive Control			37	28	484	432	435*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	76	86	72	32	34
			60	108	70	39
			89	123	83	31
			79	83	83	35
Test Chemical	Boric acid	1000	70	109	79	38	38
			72	105	83	39	38
		1800	75	92	81	36	36
			89#	94	97	36	36
		2600	71	87	90	42	41
			73	103	90	41	41
		3400	88#	90	130.5	49	43
			91	82	100	37	43
		4200	99	86	146	49	48
			107	84	150	47	48
		5000	95	79	160	56	50
			103	89	135	44	50
Positive Control	Methyl Methane Sulfonate	15	21	18	257	402	385*
Positive Control			21	17	226	367	385*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	95	109	51	18	24
			89	126	84	31
			92	85	67	24
			78	79	54	23
Test Chemical	Boric acid	1800	100	77	66	22	21
			86	92	52	20	21
		2600	115	78	64	19	22
			113	90	86	25	22
		3400	78	81	44	19	25
			85	83	78	31	25
		4200	87	64	68	26	24
			93	80	62	22	24
		5000	104	62	62	20	22
			112	55	83	25	22
Positive Control	Methyl Methane Sulfonate	15	54	31	228	140	166*
Positive Control			42	27	244	192	166*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	51	76	141	92	67
			94	109	136	48
			78	107	121	52
			81	108	185	76
Test Chemical	Boric acid	2000	62	92	107	57	51
			70	117	95	46	51
		3000	87	115	156	60	85
			56	43	184	110	85
		4000	98	116	155	53	52
			83	110	128	52	52
		5000	89	103	140	52	53
			74	90	120	54	53
Positive Control	3-Methylcholanthrene	2.5	66	72	340	171	182*
Positive Control			62	67	358	193	182*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	70	87	175	84	90
			87#	103	223.5	85
			96	104	285	99
			80	106	222	92
Test Chemical	Boric acid	1000	83#	99	211.5	85	83
			73	90	176	81	83
		2000	94#	90	278	99	98
			80	88	235	98	98
		3000	79	71	263	111	109
			80	100	257	107	109
		4000	73#	87	217.5	99	95
			100	77	273	91	95
		5000	64#	57	175.5	92	114
			57	65	230	136	114
Positive Control	3-Methylcholanthrene	2.5	59	34	867	488	536*
Positive Control			45#	38	787	583	536*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination