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Study Information
Study Attribute Value
CEBS Accession Number: 002-01743-0001-0000-8
Chemical Name: Bromodichloromethane
CASRN: 75-27-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 494532_SCE
Study Result: Equivocal

Study Result: Equivocal
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Questionable 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Test Failure 
Induced Rat Liver S9  4_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 01/23/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1035 467 0.451 9.34 26.5 0.000
Negative (Not Specified) 
0         
 50 1038 421 0.406 8.42 26.5 -10.111
Test Chemical Bromodichloromethane 
50         
 50 1024 452 0.441 9.04 26.5 -2.172 
160         
 50 1038 471 0.454 9.42 26.5 0.565 
500         
 50 1038 466 0.449 9.32 26.5 -0.503 
1600         
 0 0 0 0.000 0.00 0.0 N/A 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1032 707 0.685 14.14 26.5 51.832
Mitomycin-C 
 0.01      
 50 1028 2520 2.451 50.40 26.5 443.289
Trend: 0.060        
Probability: 0.476        

Trial #: 2    Activation: No Activation    Date: 03/06/1984    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1043 436 0.418 8.72 26.0 -0.398
Dimethyl Sulfoxide 
0         
 50 1046 439 0.420 8.78 26.0 0.000
Test Chemical Bromodichloromethane 
500         
 50 1045 427 0.409 8.54 26.0 -2.640 
1000         
 50 1047 516 0.493 10.32 26.0 17.428 
1500         
 50 1042 458 0.440 9.16 26.0 4.729 
2000         
 50 1040 518 0.498 10.36 26.0 18.676 
3000         
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1046 1402 1.340 28.04 26.0 219.362
Mitomycin-C 
 0.01      
 10 211 693 3.284 69.30 26.0 682.561
Trend: 2.956        
Probability: 0.002        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/23/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 470 0.449 9.40 26.0 0.000
Negative (Not Specified) 
0         
 50 1047 480 0.458 9.60 26.0 2.128
Test Chemical Bromodichloromethane 
50         
 50 1045 487 0.466 9.74 26.0 3.815 
160         
 50 1038 496 0.478 9.92 26.0 6.447 
500         
 50 1050 440 0.419 8.80 26.0 -6.650 
1600         
 50 1047 499 0.477 9.98 26.0 6.170 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1037 595 0.574 11.90 26.0 27.817
Cyclophosphamide 
2         
 50 1049 1192 1.136 23.84 26.0 153.133
Trend: 0.132        
Probability: 0.448        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/06/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1047 344 0.329 6.88 26.0 -19.634
Dimethyl Sulfoxide 
0         
 50 1042 426 0.409 8.52 26.0 0.000
Test Chemical Bromodichloromethane 
2000         
 50 1042 449 0.431 8.98 26.0 5.399 
3000         
 50 1043 445 0.427 8.90 26.0 4.360 
4000         
 50 1045 533 0.510 10.66 26.0 24.758 
5000         
 50 1038 511 0.492 10.22 26.0 20.415
Positive Control Cyclophosphamide 
 0.3       
 50 1043 667 0.640 13.34 26.0 56.423
Cyclophosphamide 
2         
 10 212 369 1.741 36.90 26.0 325.744
Trend: 3.707        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 06/28/1988    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 451 0.434 9.02 26.0 0.000
Test Chemical Bromodichloromethane 
1000         
 50 1042 411 0.394 8.22 26.0 -9.044 
2000         
 0 0 0 0.000 0.00 0.0 N/A 
3000         
 0 0 0 0.000 0.00 0.0 N/A 
4000         
 0 0 0 0.000 0.00 0.0 N/A 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.15      
 50 1046 581 0.555 11.62 26.0 28.086
Cyclophosphamide 
 0.6       
 10 210 196 0.933 19.60 26.0 115.225
Trend: -1.391        
Probability: 0.918        

Trial #: 4_S9    Activation: Induced Rat Liver S9    Date: 08/02/1988    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 412 0.394 8.24 26.0 0.000
Test Chemical Bromodichloromethane 
160         
 50 1040 470 0.452 9.40 26.0 14.736 
500         
 50 1041 473 0.454 9.46 26.0 15.357 
1000         
 50 1037 422 0.407 8.44 26.0 3.316 
2000         
 50 1043 454 0.435 9.08 26.0 10.511
Positive Control Cyclophosphamide 
 0.1       
 50 1049 547 0.521 10.94 26.0 32.387
Cyclophosphamide 
 0.6       
 10 208 238 1.144 23.80 26.0 190.501
Trend: 0.665        
Probability: 0.253