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Study Information
Study Attribute Value
CEBS Accession Number: 002-01743-0002-0000-9
Chemical Name: Bromodichloromethane
CASRN: 75-27-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 494532_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 03/08/1984    Harvest Time: 10.8 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Bromodichloromethane 
250         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
500         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
1000         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
2000         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 24 0.240 22.0 11 0.110 10.0 13 0.130 13.0 0 0.000 0.0
Mitomycin-C 
1         
 50 39 0.780 42.0 19 0.380 30.0 20 0.400 26.0 0 0.000 0.0
Trend: -0.994 -1.077 -0.290  
Probability: 0.840 0.859 0.614  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/07/1984    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Bromodichloromethane 
250         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
1000         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
2000         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 32 0.320 28.0 11 0.110 11.0 21 0.210 17.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 42 0.840 46.0 24 0.480 34.0 17 0.340 18.0 1 0.020 2.0
Trend: 0.290 0.000 0.290  
Probability: 0.386 0.000 0.386  


Trial #:2   Activation: No Activation    Date: 01/27/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Bromodichloromethane 
160         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
500         
 100 3 0.030 2.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
1600         
 100 4 0.040 3.0 1 0.010 1.0 3 0.030 2.0 0 0.000 0.0 
5000         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 45 0.450 32.0 20 0.200 18.0 25 0.250 21.0 0 0.000 0.0
Mitomycin-C 
1         
 100 60 0.600 45.0 30 0.300 26.0 30 0.300 25.0 0 0.000 0.0
Trend: 0.002 0.501 -0.238  
Probability: 0.499 0.308 0.594  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/26/1984    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Bromodichloromethane 
160         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 3 0.030 2.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
1600         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
5000         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 11 0.110 9.0 4 0.040 4.0 6 0.060 6.0 1 0.010 1.0
Cyclophosphamide 
50         
 100 37 0.370 31.0 13 0.130 13.0 24 0.240 22.0 0 0.000 0.0
Trend: 0.406 -0.005 0.406  
Probability: 0.342 0.502 0.342