Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-01743-0058-0000-0
Chemical Name: Bromodichloromethane
CASRN: 75-27-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 173442
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 103 59 22 33
    94 112 106 38
    61 79 81 44
    65 107 54 28
Test Chemical Bromodichloromethane 
15.625     
 64 124 61 32 41
    69 120 104 51
  
31.25      
 72 108 103 48 45
    83 119 107 43
  
62.5       
 91 90 59 22 35
    78 106 115 49
  
125         
 81 91 110 45 44
    91 71 117 43
  
250         
 69 35 86 42 47
    84 33 129 51
  
500         
   
Positive Control Methyl Methane Sulfonate 
15         
 36 18 358 328 444*
    24 7 409 560

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 110 98 85 26 26
    111 100 76 23
    99 98 74 25
    109 103 101 31
Test Chemical Bromodichloromethane 
200         
 105 83 67 21 24
    93 79 75 27
  
250         
 72 61 65 30 27
    96 59 67 23
  
300         
 105# 51 85.5 27
    148r 91 68 15
  
350         
 52 33 43 27 30
    91# 28 87 32
  
400         
 110 20 112 34 38
    82 20 106 43
Positive Control Methyl Methane Sulfonate 
15         
 40 22 165 139 172*
    41 31 252 206

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 110 226 102 89
    53 98 146 92
    70 98 148 71
    68 94 186 91
Test Chemical Bromodichloromethane 
180         
 63 69 299 159 148*
    59 67 242 136
  
240         
 41 23 233 189 165*
    60 34 255 141
  
300         
 60 29 247 138 210*
    46 15 391 283
  
360         
 51 10 459 300 271*
    56 9 402 241
  
420         
 39 4 755 651 798*
    31 4 865 945
  
480         
   
Positive Control 3-Methylcholanthrene 
2.5       
 28 22 791 953 960*
    26 19 750 968

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 129r 119 113 29 28
    110 101 101 31
    111 89 91 27
    107 110 81 25
Test Chemical Bromodichloromethane 
180         
 101 56 107 35 40
    101 40 133 44
  
240         
 79 39 100 42
    121r 32 185 51
  
300         
 95 36 135 47 47*
    112 26 156 46
  
360         
 88 20 111 42 49*
    105 21 177 56
  
420         
 92 9 219 80 76*
    99 10 216 73
  
480         
   
Positive Control 3-Methylcholanthrene 
2.5       
 89 50 666 251 266*
    81 49 680 282

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination