Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01743-0058-0000-0
Chemical Name:	Bromodichloromethane
CASRN:	75-27-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	173442
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	103	59	22	33
			94	112	106	38
			61	79	81	44
			65	107	54	28
Test Chemical	Bromodichloromethane	15.625	64	124	61	32	41
			69	120	104	51	41
		31.25	72	108	103	48	45
			83	119	107	43	45
		62.5	91	90	59	22	35
			78	106	115	49	35
		125	81	91	110	45	44
			91	71	117	43	44
		250	69	35	86	42	47
			84	33	129	51	47
		500

Positive Control	Methyl Methane Sulfonate	15	36	18	358	328	444*
Positive Control			24	7	409	560	444*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	110	98	85	26	26
			111	100	76	23
			99	98	74	25
			109	103	101	31
Test Chemical	Bromodichloromethane	200	105	83	67	21	24
			93	79	75	27	24
		250	72	61	65	30	27
			96	59	67	23	27
		300	105#	51	85.5	27
			148r	91	68	15
		350	52	33	43	27	30
			91#	28	87	32	30
		400	110	20	112	34	38
			82	20	106	43	38
Positive Control	Methyl Methane Sulfonate	15	40	22	165	139	172*
Positive Control			41	31	252	206	172*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	110	226	102	89
			53	98	146	92
			70	98	148	71
			68	94	186	91
Test Chemical	Bromodichloromethane	180	63	69	299	159	148*
			59	67	242	136	148*
		240	41	23	233	189	165*
			60	34	255	141	165*
		300	60	29	247	138	210*
			46	15	391	283	210*
		360	51	10	459	300	271*
			56	9	402	241	271*
		420	39	4	755	651	798*
			31	4	865	945	798*
		480

Positive Control	3-Methylcholanthrene	2.5	28	22	791	953	960*
Positive Control			26	19	750	968	960*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	129r	119	113	29	28
			110	101	101	31
			111	89	91	27
			107	110	81	25
Test Chemical	Bromodichloromethane	180	101	56	107	35	40
			101	40	133	44	40
		240	79	39	100	42
			121r	32	185	51
		300	95	36	135	47	47*
			112	26	156	46	47*
		360	88	20	111	42	49*
			105	21	177	56	49*
		420	92	9	219	80	76*
			99	10	216	73	76*
		480

Positive Control	3-Methylcholanthrene	2.5	89	50	666	251	266*
Positive Control			81	49	680	282	266*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination