Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01750-0018-0000-4
Chemical Name:	Butyl benzyl phthalate
CASRN:	85-68-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	811311
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	114	114	96	28	33
			74	92	67	30
			94#	122	108	38
			77#	72	85	37
Test Chemical	Butyl benzyl phthalate	5	72	77	74	34	40
			69#	82	94	45
			74#	61	87	39
		10	73#	76	92	42	40
			78	68	101	43
			92	72	98	36
		20	90	38	152	56	46
			71#	34	90	42
			79#	40	95	40
		30	52	23	141	91	77*
			63#	14	120	63	77*
		40	50	23	126	84	85*
			71	28	132	62
			54	18	173	107
		60	55	7	191	116	106*
			70	10	217	104
			59	5	176	99
Positive Control	Methyl Methane Sulfonate	5	57	33	556	327	384*
			33	18	491	501
			62	37	606	325

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	82	40	170	69	46

			93#	130	105	38
			84	130	80	32
Test Chemical	Butyl benzyl phthalate	20	57#	48	80	47	60
			58	40	63	36
			57	16	167	98
		30	75#	56	110	49	50
			91	34	128	47
			66	45	106	53
		40	71	29	125	59	58
			77	28	153	67
			69	25	99	48
		80	73#	27	183	84	79*
			39	15	103	88
			67	18	132	66
Positive Control	Methyl Methane Sulfonate	5	95	101	724	255	456*
Positive Control			37#	12	730	658	456*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	80	113	163	68	55
			82	95	137	56
			64	93	95	50
			65	99	88	45
Test Chemical	Butyl benzyl phthalate	10	54	32	91	57
		20	81	24	197	81	75
			62	22	131	70
			77	18	168	73
		30	73	13	195	89	83*
			45	11	108	80
			67	17	162	81
		40


Positive Control	Methyl Methane Sulfonate	5	35	35	584	562	563*
			35	29	616	595
			38	36	608	531

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	88	117	70	26	31
			92	94	80	29
			83	110	66	27
			67	79	82	41
Test Chemical	Butyl benzyl phthalate	10	79	72	90	38	35
			61	69	60	33	35
		20	94	39	110	39	36
			79	61	78	33	36
		30	67	36	86	43	45
			90	27	114	42
			86	39	127	49
		40	95	43	110	39	41
			65	17	68	35
			54	24	79	49
		60	99	30	102	34	40
			95	33	115	40
			101#	32	135	45
		80	68	14	106	52	52*
			60	11	94	52	52*
Positive Control	Methyl Methane Sulfonate	5	54	32	638	398	343*
			49	30	567	384
			58	26	432	248

Nonactivation Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	99	113	82	28	24
			95	99	70	24
			89	80	68	26
			105	109	62	20
Test Chemical	Butyl benzyl phthalate	10	79	72	49	21	18
			89	66	46	17
			73	48	35	16
		20	76	50	53	23	21
			87	64	54	21
			72	53	41	19
		30	92	20	57	21	21
			82	20	61	25
			95	22	49	17
		40

		50


Positive Control	Methyl Methane Sulfonate	5	83	69	791	318	295*
			88	93	780	295
			76	73	617	271

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	104	153	164	52	66
			80	69	138	57
			82	76	222	91
			104	103	195	62
Test Chemical	Butyl benzyl phthalate	30	80	86	158	66	72
			77	63	203	88
			80	76	150	63
		40	80	113	104	44	52
			78	59	149	64
			78	73	116	50
		50	79	65	101	42	50
			75	50	123	55
			83	82	128	52
		60	74	58	165	75	76
			81	67	198	81
			96	52	206	71
		80	76	55	169	74	78
			78	51	195	84
			80	62	184	77
		100	79	47	186	78	75
			73#	53	147	67
			79	58	190	80
Positive Control	3-Methylcholanthrene	2.5	41	19	683	562	550*
			49	27	768	521
			41	10	693	568

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	78	89	104	45	41
			90#	101	107	40
			86	104	73	28
			75	106	112	50
Test Chemical	Butyl benzyl phthalate	30	60	59	76	42	41
			74	64	69	31
			64	65	95	50
		40	83	66	77	31	39
			86	57	137	53
			94	81	94	33
		50	85	77	94	37	39
			96	67	122	42
			93	59	103	37
		60	71	68	89	42	40
			93	73	111	40
			82	54	92	38
		80	92	80	109	39	39
			83	69	90	36
			99	61	121	41
		100	92	42	99	36	35
			88	45	96	36
			102	60	98	32
Positive Control	3-Methylcholanthrene	2.5	64	48	687	359	309*
			76#	58	631	277
			69	50	602	291

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination