Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-01763-0011-0000-1
Chemical Name: C.I. Acid orange 10
CASRN: 1936-15-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 224313
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 91 92 96 35 38
    87 108 112 43
    94 107 100 36
    95 93 109 38
Test Chemical C.I. Acid orange 10 
312.5       
 86 110 93 36 41
    86 87 118 46
  
625         
 79 94 90 38 37
    87 96 93 35
  
1250         
 110 87 115 35 33
    100 93 95 32
  
2500         
 82 96 126 52 47
    95 92 122 43
  
5000         
 105 91 132 42 36
    116 77 101 29
Positive Control Methyl Methane Sulfonate 
15         
 55 40 193 118 123*
    55 38 213 129
Methyl Methane Sulfonate 
250         
 91 71 382 140 135*
    89 93 346 130

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 68 110 153 75 66
    69 104 152 73
    62 94 114 61
    78 92 125 53
Test Chemical C.I. Acid orange 10 
1000         
 65 126 153 78 78
    83 120 194 78
  
2000         
 78 115 149 64 65
    83 120 164 66
  
3000         
 72 108 118 55 62
    73 95 150 68
  
4000         
 60 77 183 102 77
    95 113 147 52
  
5000         
 58 77 130 74 85
    57 74 164 96
Positive Control Methyl Methane Sulfonate 
15         
 30 25 215 238 238*
    36 33 259 238
Methyl Methane Sulfonate 
250         
 53 65 591 371 354*
    58 73 588 337

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 105 95 101 32 38
    108 105 110 34
    93 94 123 44
    100 105 120 40
Test Chemical C.I. Acid orange 10 
200         
 98 115 99 34 33
    81 104 80 33
  
300         
 87 76 119 46 43
    83 80 98 40
  
500         
 81 94 104 43 43
    86 80 110 43
  
750         
 102 102 130 43 46
    83 75 125 50
  
1250         
 81 88 130 54 49
    84 84 110 44
  
2000         
   
Positive Control 3-Methylcholanthrene 
2.5       
 68 25 575 281 331*
    49 24 565 382

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 101 104 108 36 32
    87 91 77 30
    115 92 127 37
    119 113 93 26
Test Chemical C.I. Acid orange 10 
200         
 95 92 161 56 47
    95 85 108 38
  
300         
 113 100 129 38 39
    77 99 92 40
  
500         
 103 92 151 49 43
    95 97 102 36
  
750         
 103 82 174 56 46
    89 92 96 36
  
1250         
 94 65 173 62 56*
    88 80 132 50
  
2000         
   
Positive Control 3-Methylcholanthrene 
2.5       
 44 14 386 295 267*
    56 15 399 240

Induced S9 Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 70 101 136 65 61
    86 117 134 52
    73 73 136 62
    76 109 147 65
Test Chemical C.I. Acid orange 10 
500         
 76 108 143 63 70
    60 98 137 76
  
750         
 58 62 112 64 67
    74 68 154 69
  
1000         
 65 63 173 88 80
    75 73 160 71
  
1250         
 72 67 158 73 75
    78 63 177 76
  
1500         
 72 56 173 80 80
    70 46 169 80
Positive Control 3-Methylcholanthrene 
2.5       
 43 21 540 424 462*
    43 23 640 500

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 89 86 73 27 25
    89 96 62 23
    70 116 38 18
    95 102 86 30
Test Chemical C.I. Acid orange 10 
750         
 57 76 71 42 37
    70 85 67 32
  
1000         
 54 56 132 82 62*
    60 68 77 43
  
1250         
 56 55 144 86 74*
    76 50 142 62
  
1500         
 52 44 137 88 95*
    74 47 229 103
  
1750         
 66 37 231 117 104*
    71 37 193 91
  
2000         
 59 18 194 110 129*
    55 15 245 148
Positive Control 3-Methylcholanthrene 
2.5       
 20 13 392 653 559*
    35 19 481 465

Induced S9 Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 96 101 43 15 16
    81 104 32 13
    77 107 35 15
    101 88 67 22
Test Chemical C.I. Acid orange 10 
1500         
 81 66 57 23 25
    88 58 72 27
  
1750         
 91 39 84 31 35*
    78 46 90 39
  
2000         
 81 37 102 42 40*
    78 51 91 39
  
2250         
 67 20 113 56 60*
    75 25 146 65
  
2500         
 76 16 152 67 75*
    60 11 147 82
Positive Control 3-Methylcholanthrene 
2.5       
 30 14 347 381 372*
    46 18 502 362

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination