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Study Information
Study Attribute Value
CEBS Accession Number: 002-01764-0001-0000-1
Chemical Name: C.I. Acid orange 3
CASRN: 6373-74-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 835697_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/23/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 343 0.327 6.86 25.5 0.000
Test Chemical C.I. Acid orange 3 
2.4       
 50 1045 307 0.294 6.14 25.5 -10.239 
8.4       
 50 1046 336 0.321 6.72 25.5 -1.854 
25.2       
 50 1045 355 0.340 7.10 25.5 3.796 
84         
 0 0 0 0.000 0.00 32.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1050 601 0.572 12.02 25.5 74.885
Mitomycin-C 
 0.01      
 5 105 165 1.571 33.00 25.5 380.133
Trend: 0.817        
Probability: 0.207        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/23/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 339 0.323 6.78 25.5 0.000
Test Chemical C.I. Acid orange 3 
25.2       
 50 1050 443 0.422 8.86 25.5 30.678 
84         
 50 1049 735 0.701 14.70 25.5 117.021 
252         
 50 1048 891 0.850 17.82 32.5 163.333 
839.9       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1050 541 0.515 10.82 25.5 59.587
Cyclophosphamide 
2         
 5 105 152 1.448 30.40 25.5 348.378
Trend: 17.810        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/07/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 525 180 0.343 7.20 25.5 0.000
Test Chemical C.I. Acid orange 3 
76         
 25 524 269 0.513 10.76 25.5 49.730 
126.7       
 25 519 514 0.990 20.56 32.5 188.857 
253.3       
 25 519 327 0.630 13.08 32.5 83.767 
402         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 25 525 339 0.646 13.56 25.5 88.333
Cyclophosphamide 
2         
 5 104 192 1.846 38.40 25.5 438.462
Trend: 8.004        
Probability: 0.000