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Study Information
Study Attribute Value
CEBS Accession Number: 002-01764-0002-0000-2
Chemical Name: C.I. Acid orange 3
CASRN: 6373-74-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 835697_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 09/11/1986    Harvest Time: 20.2 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 5 0.050 3.0 3 0.030 3.0 2 0.020 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 4 0.040 4.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: C.I. Acid orange 3 
60.6       
 100 8 0.080 4.0 1 0.010 1.0 6 0.060 3.0 1 0.010 1.0 
90.5       
 100 56 0.560 30.0 29 0.290 19.0 25 0.250 15.0 2 0.020 2.0 
120.6       
 100 93 0.930 39.0 19 0.190 16.0 54 0.540 27.0 20 0.200 2.0 
180         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 19 0.190 16.0 10 0.100 10.0 6 0.060 4.0 3 0.030 3.0
Mitomycin-C 
 0.08      
 25 19 0.760 48.0 9 0.360 28.0 9 0.360 24.0 1 0.040 4.0
Trend: 7.464 4.150 6.527  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 08/21/1986    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 7 0.035 3.5 2 0.010 1.0 4 0.020 2.0 1 0.005 0.5
Test Chemical: C.I. Acid orange 3 
44.6       
 200 7 0.035 3.5 4 0.020 2.0 2 0.010 1.0 1 0.005 0.5 
60         
 200 5 0.025 2.5 1 0.005 0.5 2 0.010 1.0 2 0.010 1.0 
148.5       
 200 13 0.065 5.0 9 0.045 4.0 3 0.015 1.0 1 0.005 0.5 
300         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 200 25 0.125 11.5 9 0.045 4.5 16 0.080 7.5 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 23 0.920 56.0 9 0.360 28.0 14 0.560 40.0 0 0.000 0.0
Trend: 0.800 2.130 -0.819  
Probability: 0.212 0.017 0.794  


Trial #:2   Activation: No Activation    Date: 08/21/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 1 0.005 0.5 2 0.010 1.0 1 0.005 0.5
Dimethyl Sulfoxide 
0         
 200 5 0.025 2.5 2 0.010 1.0 2 0.010 1.0 1 0.005 0.5
Test Chemical: C.I. Acid orange 3 
60         
 200 11 0.055 4.5 7 0.035 3.0 3 0.015 1.0 1 0.005 0.5 
89.1       
 50 101 2.020 66.0 14 0.280 26.0 66 1.320 50.0 21 0.420 6.0 
120         
 50 68 1.360 60.0 3 0.060 6.0 54 1.080 54.0 11 0.220 4.0
Positive Control: Mitomycin-C 
 0.05      
 200 27 0.135 13.0 18 0.090 9.0 9 0.045 4.5 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 17 0.680 44.0 6 0.240 24.0 11 0.440 28.0 0 0.000 0.0
Trend: 12.803 4.550 12.597  
Probability: 0.000 0.000 0.000