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Study Information
Study Attribute Value
CEBS Accession Number: 002-01765-0001-0000-2
Chemical Name: C.I. Acid red 114
CASRN: 6459-94-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 226009_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Negative 
No Activation  3  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Test Failure 

Trial #: 1    Activation: No Activation    Date: 10/10/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1039 406 0.391 8.12 26.0 2.010
Medium 
0         
 50 1039 398 0.383 7.96 26.0 0.000
Test Chemical C.I. Acid red 114 
 0.16      
 50 1041 384 0.369 7.68 26.0 -3.703 
 0.5       
 50 1030 514 0.499 10.28 26.0 30.274 
5         
 50 1042 511 0.490 10.22 26.0 28.022 
16         
 50 1038 446 0.430 8.92 26.0 12.168 
50         
 50 1035 483 0.467 9.66 26.0 21.826 
160         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.01      
 50 1037 2209 2.130 44.18 26.0 456.096
Trend: 3.372        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/20/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1034 398 0.385 7.96 26.0 -3.865
Medium 
0         
 50 1034 414 0.400 8.28 26.0 0.000
Test Chemical C.I. Acid red 114 
10         
 50 1037 455 0.439 9.10 26.0 9.585 
25         
 50 1027 453 0.441 9.06 26.0 10.166 
50         
 50 1032 456 0.442 9.12 26.0 10.358 
75         
 0 0 0 0.000 0.00 0.0 N/A 
100         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1047 1430 1.366 28.60 26.0 241.122
Mitomycin-C 
 0.01      
 50 1046 1854 1.772 37.08 26.0 342.689
Trend: 1.322        
Probability: 0.093        

Trial #: 3    Activation: No Activation    Date: 12/04/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1033 418 0.405 8.36 26.0 0.000
Negative (Not Specified) 
0         
 50 1036 457 0.441 9.14 26.0 9.014
Test Chemical C.I. Acid red 114 
 0.5       
 50 1043 423 0.406 8.46 26.0 0.226 
1         
 50 1041 463 0.445 9.26 26.0 9.914 
5         
 50 1036 449 0.433 8.98 26.0 7.105 
10         
 50 1040 469 0.451 9.38 26.0 11.446 
25         
 50 1030 442 0.429 8.84 26.0 6.050 
50         
 50 1041 429 0.412 8.58 26.0 1.843
Positive Control Mitomycin-C 
 0.01      
 50 1035 2148 2.075 42.96 26.0 412.883
Trend: 0.732        
Probability: 0.232        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/10/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1042 468 0.449 9.36 26.0 -7.310
Medium 
0         
 50 1036 502 0.485 10.04 26.0 0.000
Test Chemical C.I. Acid red 114 
 0.5       
 50 1041 499 0.479 9.98 26.0 -1.075 
1.6       
 50 1031 524 0.508 10.48 26.0 4.889 
5         
 50 1034 507 0.490 10.14 26.0 1.191 
16         
 50 1037 411 0.396 8.22 26.0 -18.206 
50         
 50 1034 493 0.477 9.86 26.0 -1.603 
160         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
1.5       
 50 1042 1723 1.654 34.46 26.0 241.251
Trend: -1.716        
Probability: 0.957        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 10/29/1982    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1037 402 0.388 8.04 26.0 -1.749
Medium 
0         
 50 1029 406 0.395 8.12 26.0 0.000
Test Chemical C.I. Acid red 114 
5         
 50 1026 443 0.432 8.86 50.0 9.432 
10         
 50 1034 441 0.426 8.82 50.0 8.095 
25         
 50 1011 481 0.476 9.62 50.0 20.582 
50         
 50 1026 526 0.513 10.52 50.0 29.935 
75         
 0 0 0 0.000 0.00 0.0 N/A 
100         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
1.5       
 50 1017 1763 1.734 35.26 26.0 339.360
Trend: 4.270        
Probability: 0.000