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Study Information
Study Attribute Value
CEBS Accession Number: 002-01765-0002-0000-3
Chemical Name: C.I. Acid red 114
CASRN: 6459-94-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 226009_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  Negative 
Induced Rat Liver S9  2_S9  Test Failure 
No Activation  Test Failure 

Trial #:1   Activation: No Activation    Date: 12/04/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Medium
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: C.I. Acid red 114
 0.5       
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
1         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
5         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
10         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
25         
100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
50         
100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.25      
100 33 0.330 25.0 9 0.090 9.0 20 0.200 19.0 4 0.040 3.0
Trend: 1.139 1.712 0.261  
Probability: 0.127 0.043 0.397  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/12/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Medium
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: C.I. Acid red 114
 0.5       
100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
1.6       
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
5         
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
16         
100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
50         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
160         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
25         
100 48 0.480 31.0 16 0.160 12.0 25 0.250 18.0 7 0.070 6.0
Trend: -0.133 -0.294 0.291  
Probability: 0.553 0.616 0.386  


Trial #:2   Activation: No Activation    Date: 10/12/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Medium
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified)
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: C.I. Acid red 114
1.6       
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
5         
100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0
16         
100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
50         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
160         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.5       
100 52 0.520 31.0 20 0.200 16.0 30 0.300 21.0 2 0.020 2.0
Trend: 1.003 0.501 0.865  
Probability: 0.158 0.308 0.193  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 10/26/1982    Harvest Time: 12 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Medium
0         
100 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified)
0         
100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: C.I. Acid red 114
50         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
100         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
200         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
300         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
400         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
500         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
25         
100 76 0.760 47.0 39 0.390 30.0 34 0.340 27.0 3 0.030 3.0
Trend: -1.745 -1.745 0.000  
Probability: 0.960 0.960 0.000  


Trial #:3   Activation: No Activation    Date: 10/26/1982    Harvest Time: 12 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Medium
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: C.I. Acid red 114
100         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
200         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
300         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
400         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
500         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.5       
100 47 0.470 34.0 11 0.110 8.0 20 0.200 17.0 16 0.160 13.0
Trend: 0.000 0.000 0.000  
Probability: 0.000 0.000 0.000