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Study Information
Study Attribute Value
CEBS Accession Number: 002-01766-0010-0000-3
Chemical Name: C.I. Acid red 14
CASRN: 3567-69-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 561870
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 86 104 71 28 27
    101 101 88 29
    87 103 71 27
    87 92 65 25
Test Chemical C.I. Acid red 14 
300.75      
 84 83 56 22 31
    92 104 111 40
  
601.5       
 88 100 71 27 25
    85 97 59 23
  
1203         
 97 104 61 21 24
    83 93 65 26
  
2406         
 80 93 69 29 29
    89 100 78 29
  
4812         
 84 92 58 23 22
    79 91 49 21
Positive Control Methyl Methane Sulfonate 
15         
 61 42 157 86 78*
    58 38 125 71
Methyl Methane Sulfonate 
250         
 97# 91 459 158 196*
    58 63 407 234

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 76 96 111 49 37
    66 91 80 41
    55 102 44 27
    71 111 67 32
Test Chemical C.I. Acid red 14 
1000         
 71 102 61 29 31
    73 103 72 33
  
2000         
 58 80 107 62 55
    51 68 74 48
  
3000         
 60 86 78 43 40
    64 89 69 36
  
4000         
 62 75 65 35 37
    54 69 64 40
  
5000         
 55 61 53 32 35
    50 66 58 39
Positive Control Methyl Methane Sulfonate 
15         
 40 41 62 52 65*
    37 34 87 78
Methyl Methane Sulfonate 
250         
 67 80 276 138 127*
    73 82 252 116

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 108 113 49 15 25
    68 91 53 26
    78 97 85 36
    90 100 62 23
Test Chemical C.I. Acid red 14 
1000         
 76 107 69 30 25
    95 139 56 20
  
2000         
 69 93 53 26 32
    60 83 67 37
  
3000         
 52 75 39 25 25
    79 116 59 25
  
4000         
 57 96 49 29 27
    68 108 52 26
  
5000         
 77 90 64 28 36
    69 102 93 45
Positive Control 3-Methylcholanthrene 
2.5       
 38 31 209 184 202*
    43 30 284 219

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 76 106 100 44 42
    78 69 98 42
    85 123 85 33
    65 103 95 49
Test Chemical C.I. Acid red 14 
1000         
 78 152 87 37 36
    98 163 101 34
  
2000         
 88 163 120 45 50
    79 143 131 55
  
3000         
 89 142 108 40 37
    95 187 95 33
  
4000         
 82 164 105 43 48
    80 149 127 53
  
5000         
 80 112 141 59 52
    86 171 114 44
Positive Control 3-Methylcholanthrene 
2.5       
 70 54 359 172 180*
    66 49 376 189

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination