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Study Information
Study Attribute Value
CEBS Accession Number: 002-01768-0001-0000-5
Chemical Name: C.I. Acid yellow 73 (fluorescein sodium)
CASRN: 518-47-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 372823_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Weakly Positive 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 03/26/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1048 308 0.294 6.16 26.5 0.000
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
5.01      
 50 1044 317 0.304 6.34 26.5 3.316 
15         
 50 1046 309 0.295 6.18 26.5 0.517 
50.1       
 50 1048 441 0.421 8.82 30.5 43.182
Positive Control Mitomycin-C 
 0.0015    
 50 1046 476 0.455 9.52 26.5 54.841
Mitomycin-C 
 0.01      
 10 210 252 1.200 25.20 26.5 308.312
Trend: 4.766        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 04/18/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1044 289 0.277 5.78 26.0 0.000
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
75         
 50 1047 318 0.304 6.36 26.0 9.719 
99.8       
 50 1047 344 0.329 6.88 26.0 18.690 
150         
 50 1044 396 0.379 7.92 30.0 37.024
Positive Control Mitomycin-C 
 0.0015    
 50 1051 516 0.491 10.32 26.5 77.358
Mitomycin-C 
 0.01      
 10 209 230 1.100 23.00 26.5 297.543
Trend: 4.267        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 03/26/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1046 415 0.397 8.30 25.5 0.000
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
150         
 50 1050 506 0.482 10.12 25.5 21.463 
501         
 50 1050 783 0.746 15.66 25.5 87.956 
1500         
 50 1047 924 0.883 18.48 29.5 122.438
Positive Control Cyclophosphamide 
 0.4       
 50 1048 795 0.759 15.90 25.5 91.201
Cyclophosphamide 
2.5       
 10 211 440 2.085 44.00 25.5 425.598
Trend: 15.758        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 05/07/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1046 395 0.378 7.90 25.5 0.000
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
134         
 50 1055 813 0.771 16.26 25.5 104.067 
267         
 50 1052 939 0.893 18.78 25.5 136.366 
445         
 50 1039 1115 1.073 22.30 29.9 184.180 
668         
 50 1044 1154 1.105 23.08 29.5 192.712
Positive Control Cyclophosphamide 
 0.4       
 50 1046 1097 1.049 21.94 25.5 177.722
Cyclophosphamide 
2.5       
 10 209 684 3.273 68.40 25.5 766.651
Trend: 19.780        
Probability: 0.000