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Study Information
Study Attribute Value
CEBS Accession Number: 002-01768-0006-0000-0
Chemical Name: C.I. Acid yellow 73 (fluorescein sodium)
CASRN: 518-47-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 099061
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 103 94 210 68 58
    129r 112 205 53
    109# 108 194 59
    97 98 140 48
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
250         
 101 57 177 58 74
    91 58 178 65
    93 47 270 97
  
500         
 106 35 247 78 73
    91 25 230 84
    91# 29 151 55
  
1000         
 52 13 236 151 107*
    109 19 220 67
    97 19 298 102
  
2000         
 56# 4 201 119 145*
    56 8 300 178
    42 5 174 139
  
3000         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 75# 35 435 193 253*
    83 47 614 246
    72 34 687 320

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 115 124 133 38 45
    122r 80 142 39
    107 69 155 48
    84 107 124 49
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
50         
 107 124 122 38 40
    102 104 115 37
    104 110 143 46
  
100         
 102# 55 127 42 40
    87 89 97 37
    110 107 133 40
  
200         
 110 30 137 41 48
    85 33 153 60
    89 18 113 42
  
400         
 85 16 257 101 81*
    112 63 219 65
    83# 13 193 78
  
600         
 90# 13 179 67 68*
    111 28 197 59
    94 14 224 79
Positive Control Methyl Methane Sulfonate 
5         
 43 20 833 651 551*
    60 28 810 449
    52 30 868 553

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 104 111 103 33 35
    92 99 98 36
    81 108 88 36
    74 82 75 34
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
200         
 58 26 143 83 68*
    83 46 147 59
    79# 48 147 62
  
400         
 88 38 170 64 77*
    56 24 171 102
    81 26 159 65
  
500         
 72 21 158 74 63*
    77 28 139 60
    90# 29 146 54
  
600         
 119 23 262 73 62*
    92 23 163 59
    117 32 190 54
  
800         
 96 11 271 95 80*
    106 22 200 63
    109 18 267 82
  
1000         
 73 10 216 99 79*
    104 18 187 60
    120r 18 227 63
Positive Control Methyl Methane Sulfonate 
5         
 63 33 626 334 270*
    70# 49 434 207
    60 27 483 270

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 119 100 264 74 82
    80 85 204 86
    94 106 254 90
    104 110 245 79
Test Chemical C.I. Acid yellow 73 (fluorescein sodium) 
31.3       
 83 87 178 72 76
    112 69 236 71
    72 67 184 85
  
62.5       
 85 57 195 76 80
    101 54 266 88
    102 55 236 77
  
125         
 91 44 242 88 95
    97 49 294 101
    124r 68 230 62
  
250         
 104 28 280 90 96
    85 20 253 99
    85 16 251 98
  
500         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 35 14 901 871 895*
    58 32 1244 711
    26 10 873 1105

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination