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Study Information
Study Attribute Value
CEBS Accession Number: 002-01771-0010-0000-9
Chemical Name: C.I. Basic red 9 monohydrochloride
CASRN: 569-61-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G61721
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 97 118 40 38
    96 103 104 36
Test Chemical C.I. Basic red 9 monohydrochloride 
 0.625     
 97 84 81 28 35
    102# 79 130.5 43
  
1.25      
 73 50 90 41 44
    99 57 139 47
  
2.5       
 94 50 88 31 34
    90 51 99 37
  
5         
 87 33 127 49 44
    85# 30 100 39
  
7.5       
 25 7 65 86 78*
    34 9 71 70
  
10         
 36 4 71 66 89*
    14 2 48 113
Positive Control Ethyl Methane Sulfonate 
500         
 63 48 1020 538 559*
    56# 42 978 580

Nonactivation Trial: 2 Experiment Call: Not a Valid Test
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 123 26 11 16
    62 85 34 18
    62 81 38 20
    75 111 30 13
Test Chemical C.I. Basic red 9 monohydrochloride 
 0.625     
 71 110 30 14 19
    77 96 56 24
  
1.25      
 87 98 64 25 23
    66 44 43 22
  
2.5       
 68# 64 48 23 23
    66 49 45 23
  
5         
 64 42 54 28 25
    55 39 34 21
  
7.5       
 48 23 53 37 31*
    54 29 39 24
  
10         
 61 23 52 29 29*
    38 9 33 29
Positive Control Ethyl Methane Sulfonate 
500         
 29 22 756 874 788*
    31 25 645 701

Nonactivation Trial: 3 Experiment Call: Not a Valid Test
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 58 82 68 39 45
    92 135 103 38
    62 83 111 60
    44r 81 87 66
Test Chemical C.I. Basic red 9 monohydrochloride 
2.5       
 84 72 90 36 88*
    27 24 113 141
  
5         
 66 44 70 35 38
    112 76 125 37
    79 51 96 41
  
6         
 71 38 97 45 47
    66 34 97 49
    66 40 91 46
  
7         
 63 22 95 51 52
    50 26 98 66
    56 20 68 40
  
8         
 57 24 81 48 42
    49 17 58 40
    64 25 75 39
  
9         
 48 10 90 63 54
    49 12 56 38
    55 22 100 61
  
10         
 19 6 111 191 176*
    13 3 50 130
    12 3 76 205
Positive Control Ethyl Methane Sulfonate 
500         
 35 22 878 828 814*
    36 26 977 905
    45 42 948 710

Nonactivation Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 96 95 38 26
    69 102 48 23
    69 107 35 17
    82 94 64 26
Test Chemical C.I. Basic red 9 monohydrochloride 
1         
 71 22 52 24 28
    60 20 41 23
    63 21 69 37
  
2         
 68 14 63 31 32
    56 17 57 34
    63 16 61 32
  
4         
 41 7 57 47 49*
    39# 5 40.5 35
    27 4 53 66
  
6         
 10r 1 27 89
    7r 0 42 205
    7r 1 30 154
  
8         
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 19 8 1067 1905 1786*
    21 12 1028 1619
    20 8 1091 1834

Nonactivation Trial: 5 Experiment Call: Not a Valid Test
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 96 68 29 22
    67 103 41 20
    67 108 29 14
    78 93 54 23
Test Chemical C.I. Basic red 9 monohydrochloride 
1         
 69 22 38 18 21
    61 21 48 26
    58 20 30 17
  
2         
 65 14 45 23 23
    54 16 35 22
    62 16 47 25
  
4         
 40 7 38 31 34*
    36# 5 25.5 24
    26 4 36 47
  
6         
 9r 1 20 71
    6r 0 22 129
    6r 1 19 109
  
8         
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 18 8 985 1791 1701*
    20 11 936 1600
    19 8 967 1712

Non-Induced S9 Trial: 1 Experiment Call: Not a Valid Test
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106 104 101 32 36
    101 108 104 34
    108 98 155 48
    100 90 93 31
Test Chemical C.I. Basic red 9 monohydrochloride 
7.8       
 97 75 80 28
    123r 98 109 30
  
15.6       
 82 62 90 37 32
    93 61 74 27
  
31.3       
 101 51 82 27 33
    83 38 96 39
  
62.5       
 82 28 80 33 38
    64 21 85 44
  
125         
 7r 1 18 90
    4r 0 15 125
  
250         
   
Positive Control 3-Methylcholanthrene 
7         
 89 84 399 150 177*
    77 57 473 204

Non-Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 94 149 57 58
    84 99 135 54
    92 99 191 69
    95 108 154 54
Test Chemical C.I. Basic red 9 monohydrochloride 
18.8       
 83# 85 130.5 53 54
    81 72 133 55
  
25         
 99 68 124 42 48
    85 52 140 55
  
37.5       
 99 38 139 47 47
    100 51 143 48
  
50         
 96 34 96 33 36
    104 43 120 38
  
75         
 79# 28 100.5 43 44
    107 28 148 46
  
100         
 71# 13 79.5 38 33
    23 6 19 28
Positive Control 3-Methylcholanthrene 
7         
 89 52 436 164 180*
    81 55 478 197

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 100 185 70 64
    95 98 204 71
    96 72 171 60
    88 130 144 54
Test Chemical C.I. Basic red 9 monohydrochloride 
7.8       
 132r 121 196 49
    84 64 193 76
  
15.6       
 84 61 158 63 57
    83 63 128 52
  
31.3       
 78 36 158 68 65
    79 30 145 62
  
62.5       
 50 11 123 82 79
    36 8 82 75
  
187         
   
Positive Control 3-Methylcholanthrene 
5         
 63 23 854 449 459*
    43 20 607 469

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 114 35 14 17
    91 125 46 17
    60 60 38 21
Test Chemical C.I. Basic red 9 monohydrochloride 
6.25      
 109 92 59 18 21
    95 82 49 17
    97 88 83 29
  
12.5       
 86 61 32 12 18
    76 62 53 23
    121r 63 88 24
  
25         
 82 39 47 19 20
    101 44 57 19
    80 40 50 21
  
50         
 86 22 57 22 21
    70 21 40 19
    66 18 46 23
  
75         
 37 7 18 16 22
    44 9 32 25
    43 6 33 26
  
100         
 14 2 19 46 47*
    11 2 17 54
    23 4 27 40
Positive Control 3-Methylcholanthrene 
5         
 41 20 287 232 235*
    52 28 357 228
    52 23 378 244

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination