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Study Information
Study Attribute Value
CEBS Accession Number: 002-01771-0011-0000-0
Chemical Name: C.I. Basic red 9 monohydrochloride
CASRN: 569-61-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G90579
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 98 70 27 28
    101 102 86 28
Test Chemical C.I. Basic red 9 monohydrochloride 
 0.77      
 94 68 78 28 27
    74 54 58 26
  
1.5       
 71 36 104 49 44
    72 40 83 39
  
3.07      
 43 19 48 38 45
    42 15 66 52
  
6.1       
 15 3 33 75 67*
    15 3 27 60
  
7.7       
 7r 1 13 60
    5r 1 18 116
  
9.6       
 2r 0 16 267
Positive Control Ethyl Methane Sulfonate 
500         
 54 42 801 498 470*
    60 47 800 443

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 105 62 24 22
    81 95 48 20
Test Chemical C.I. Basic red 9 monohydrochloride 
 0.512     
 104 105 107 34
    124r 121 131 35
  
1.02      
 61 49 146 79 59*
    99 81 112 38
  
2.05      
 83 49 94 38 41*
    74 43 100 45
  
4.1       
 20 7 67 110 100*
    27 10 72 90
  
5.12      
 12 3 60 174
    10r 3 17 58
  
6.4       
 3r 1 4 50
    5r 1 0 0
  
8         
 2r 0 0 0
    2r 0 0 0
  
10         
 1r 0 0 0
    2r 0 8 123
Positive Control Ethyl Methane Sulfonate 
500         
 52 41 424 271 290*
    48 38 444 309

Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 117 311 142 161
    63 83 337 180
Test Chemical C.I. Basic red 9 monohydrochloride 
17.2       
 63 133 217 115 103
    79 129 215 90
  
24.6       
 78 117 254 108 106
    78 100 242 104
  
35.1       
 71 80 287 134 134
    71 90 287 134
  
50.2       
 45 29 274 204 235
    37 25 295 267
  
71.7       
 13 5 279 697 622*
    14 4 227 547
  
102         
 1r 0 207 8280
    6r 1 237 1354
  
128         
   
Positive Control 3-Methylcholanthrene 
5         
 20 11 307 507 489*
    19 8 261 470

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 99 368 141 144
    81 101 358 147
Test Chemical C.I. Basic red 9 monohydrochloride 
16.8       
 114 108 372 108 128
    95 98 419 147
  
21         
 103 91 440 143 141
    98 86 407 139
  
26.2       
 85 66 428 168 171
    88 70 459 174
  
32.8       
 83 53 459 184
  
41         
 61 32 422 232 231*
    73 43 505 231
  
51.2       
 55 26 382 230
  
64         
 46 14 262 188 199
    36 10 227 210
  
80         
 15 3 164 357 312*
    19 4 148 267
Positive Control 3-Methylcholanthrene 
5         
 30 13 480 530 500*
    33 16 470 470

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 56 75 106 64 50
    78 101 93 40
    87 112 105 40
    95 111 156 55
Test Chemical C.I. Basic red 9 monohydrochloride 
33         
 75 54 154 68
    144r 95 99 23
  
41         
 69 41 253 123 108*
    87 54 243 93
  
51         
 52 26 284 180 208*
    64 30 455 236
  
64         
 47 18 278 196 211*
    24 9 163 226
  
80         
 14 4 56 138 576*
    18 6 552 1013
  
100         
 1r 0 41 1367
    2r 0 123 2460
Positive Control 3-Methylcholanthrene 
5         
 38 32 338 295 297*
    57 47 403 234
    38 25 412 361

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 102 322 128 102
    88 107 220 83
    91 109 219 81
    68 82 240 118
Test Chemical C.I. Basic red 9 monohydrochloride 
24         
 84 51 277 111 119
    76 51 291 127
  
29         
 89 49 286 108 116
    83 45 309 124
  
37         
 69 31 336 162 156
    68 33 306 150
  
46         
 65 24 281 144 145
    75 29 330 146
  
58         
 48 15 293 203 196*
    57 18 321 188
  
72         
 10 2 192 619 598*
    13 3 225 577
  
90         
 4r 1 86 662
    2r 0 52 867
Positive Control 3-Methylcholanthrene 
5         
 81 65 905 375 397*
    78 65 959 412
    77 62 930 404

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination