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Study Information
Study Attribute Value
CEBS Accession Number: 002-01779-0001-0000-7
Chemical Name: C.I. Direct blue 15
CASRN: 2429-74-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 279945_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 01/03/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1043 443 0.425 8.86 25.8 0.000
Test Chemical C.I. Direct blue 15 
250         
 50 1035 435 0.420 8.70 25.8 -1.047 
500         
 50 1034 423 0.409 8.46 25.8 -3.684 
750         
 50 1034 439 0.425 8.78 25.8 -0.040
Positive Control Mitomycin-C 
 0.005     
 25 520 1306 2.512 52.24 25.8 491.317
Trend: -0.187        
Probability: 0.574        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/15/1981    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1052 576 0.548 11.52 25.3 0.316
Medium 
0         
 50 1037 566 0.546 11.32 26.3 0.000
Test Chemical C.I. Direct blue 15 
83.3       
 50 1041 545 0.524 10.90 26.3 -4.080 
833         
 50 1038 658 0.634 13.16 26.3 16.142 
2500         
 50 1039 570 0.549 11.40 26.3 0.513
Positive Control Cyclophosphamide 
1.5       
 50 1039 2675 2.575 53.50 26.3 371.705
Trend: 1.555        
Probability: 0.060        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/26/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1044 468 0.448 9.36 25.5 0.000
Test Chemical C.I. Direct blue 15 
2000         
 50 1033 530 0.513 10.60 25.5 14.454 
2250         
 50 1024 515 0.503 10.30 25.5 12.192 
2500         
 50 1030 488 0.474 9.76 25.5 5.691
Positive Control Cyclophosphamide 
1.5       
 25 514 980 1.907 39.20 25.5 325.322
Trend: 0.713        
Probability: 0.238