COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01779-0002-0000-8
Chemical Name: C.I. Direct blue 15
CASRN: 2429-74-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 279945_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 01/12/1982    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Medium 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: C.I. Direct blue 15 
1500         
 100 8 0.080 7.0 5 0.050 5.0 3 0.030 3.0 0 0.000 0.0 
1750         
 100 16 0.160 10.0 7 0.070 7.0 7 0.070 4.0 2 0.020 2.0 
2000         
 100 8 0.080 6.0 6 0.060 4.0 1 0.010 1.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.5       
 50 25 0.500 34.0 17 0.340 13.0 8 0.160 8.0 0 0.000 0.0
Trend: 1.125 1.254 -0.254  
Probability: 0.130 0.105 0.600  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 01/12/1982    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Medium 
0         
 100 6 0.060 5.0 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0
Test Chemical: C.I. Direct blue 15 
2000         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
2250         
 100 9 0.090 7.0 2 0.020 2.0 5 0.050 4.0 2 0.020 2.0 
2500         
 100 10 0.100 9.0 4 0.040 4.0 5 0.050 4.0 1 0.010 1.0
Positive Control: Cyclophosphamide 
25         
 50 18 0.360 24.0 4 0.080 6.0 12 0.240 18.0 2 0.040 4.0
Trend: 1.385 0.562 0.888  
Probability: 0.083 0.287 0.187  


Trial #:2   Activation: No Activation    Date: 01/26/1982    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 6 0.060 5.0 2 0.020 2.0 4 0.040 3.0 0 0.000 0.0
Medium 
0         
 100 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 1 0.010 1.0
Test Chemical: C.I. Direct blue 15 
1750         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
2000         
 100 2 0.020 2.0 0 0.000 0.0 1 0.010 1.0 1 0.010 1.0 
2250         
 100 5 0.050 5.0 1 0.010 1.0 4 0.040 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 50 15 0.300 26.0 7 0.140 12.0 8 0.160 14.0 0 0.000 0.0
Trend: 1.943 0.610 2.593  
Probability: 0.026 0.271 0.005  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/26/1982    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0
Medium 
0         
 100 4 0.040 4.0 1 0.010 1.0 2 0.020 2.0 1 0.010 1.0
Test Chemical: C.I. Direct blue 15 
2000         
 100 11 0.110 7.0 1 0.010 1.0 9 0.090 6.0 1 0.010 1.0 
2250         
 100 4 0.040 4.0 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 
2500         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 35 0.700 36.0 12 0.240 20.0 12 0.240 18.0 11 0.220 4.0
Trend: -0.995 0.217 -1.163  
Probability: 0.840 0.414 0.878