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Study Information
Study Attribute Value
CEBS Accession Number: 002-01781-0001-0000-0
Chemical Name: C.I. Direct blue 218
CASRN: 28407-37-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 376714_SCE
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Weakly Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Questionable 

Trial #: 1    Activation: No Activation    Date: 10/10/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1039 398 0.383 7.96 26.0 0.000
Negative (Not Specified) 
0         
 50 1039 406 0.391 8.12 26.0 2.010
Test Chemical C.I. Direct blue 218 
 0.16      
 50 1035 424 0.410 8.48 26.0 6.944 
5         
 50 1033 440 0.426 8.80 26.0 11.195 
16         
 50 1038 461 0.444 9.22 26.0 15.941 
50         
 50 1043 426 0.408 8.52 26.0 6.625 
160         
 50 1038 457 0.440 9.14 26.0 14.935
Positive Control Mitomycin-C 
 0.01      
 50 1037 2209 2.130 44.18 26.0 456.096
Trend: 1.955        
Probability: 0.025        

Trial #: 2    Activation: No Activation    Date: 10/20/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 398 0.381 7.96 26.0 -4.786
Medium 
0         
 50 1034 414 0.400 8.28 26.0 0.000
Test Chemical C.I. Direct blue 218 
25         
 50 1036 426 0.411 8.52 26.0 2.700 
50         
 50 1039 414 0.398 8.28 26.0 -0.481 
75         
 50 1038 404 0.389 8.08 26.0 -2.792 
100         
 50 1043 488 0.468 9.76 26.0 16.857 
200         
 50 1034 561 0.543 11.22 26.0 35.507 
300         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1047 1430 1.366 28.60 26.0 241.122
Mitomycin-C 
 0.01      
 50 1046 1854 1.772 37.08 26.0 342.689
Trend: 4.905        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/10/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1036 502 0.485 10.04 26.0 0.000
Negative (Not Specified) 
0         
 50 1042 468 0.449 9.36 26.0 -7.310
Test Chemical C.I. Direct blue 218 
1.6       
 50 1042 426 0.409 8.52 26.0 -15.628 
5         
 50 1046 434 0.415 8.68 26.0 -14.372 
16         
 50 1046 401 0.383 8.02 26.0 -20.883 
50         
 50 1042 467 0.448 9.34 26.0 -7.508 
160         
 50 1035 509 0.492 10.18 26.0 1.492
Positive Control Cyclophosphamide 
1.5       
 50 1042 1723 1.654 34.46 26.0 241.251
Trend: 0.715        
Probability: 0.237        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 10/29/1982    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1029 406 0.395 8.12 26.0 0.000
Negative (Not Specified) 
0         
 50 1037 402 0.388 8.04 26.0 -1.749
Test Chemical C.I. Direct blue 218 
25         
 50 1030 412 0.400 8.24 26.0 1.379 
50         
 50 1029 435 0.423 8.70 26.0 7.143 
100         
 50 1031 438 0.425 8.76 26.0 7.672 
150         
 50 1027 465 0.453 9.30 26.0 14.755 
200         
 50 1007 454 0.451 9.08 26.0 14.266
Positive Control Cyclophosphamide 
1.5       
 50 1017 1763 1.734 35.26 26.0 339.360
Trend: 2.591        
Probability: 0.005