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Study Information
Study Attribute Value
CEBS Accession Number: 002-01787-0002-0000-7
Chemical Name: C.I. Direct brown 95
CASRN: 16071-86-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 754489_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 
Induced Rat Liver S9  3_S9  Negative 

Trial #:1   Activation: No Activation    Date: 02/24/1982    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Water 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: C.I. Direct brown 95 
12         
 100 6 0.060 6.0 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 
38         
 100 7 0.070 5.0 0 0.000 0.0 7 0.070 5.0 0 0.000 0.0 
120         
 100 6 0.060 5.0 0 0.000 0.0 6 0.060 5.0 0 0.000 0.0 
380         
 100 9 0.090 7.0 1 0.010 1.0 8 0.080 7.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.15      
 100 38 0.380 25.0 10 0.100 10.0 28 0.280 20.0 0 0.000 0.0
Trend: 0.997 -0.355 1.510  
Probability: 0.159 0.639 0.066  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 09/16/1982    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Water 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: C.I. Direct brown 95 
200         
 100 5 0.050 5.0 1 0.010 1.0 4 0.040 4.0 0 0.000 0.0 
250         
 100 10 0.100 8.0 3 0.030 3.0 7 0.070 6.0 0 0.000 0.0 
300         
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0 
350         
 100 8 0.080 7.0 1 0.010 1.0 7 0.070 6.0 0 0.000 0.0 
400         
 100 10 0.100 8.0 1 0.010 1.0 9 0.090 7.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 21 0.210 18.0 6 0.060 5.0 15 0.150 13.0 0 0.000 0.0
Trend: 1.421 0.535 1.249  
Probability: 0.078 0.296 0.106  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 05/19/1982    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Water 
0         
 100 8 0.080 6.0 2 0.020 2.0 6 0.060 4.0 0 0.000 0.0
Test Chemical: C.I. Direct brown 95 
100         
 100 9 0.090 5.0 1 0.010 1.0 8 0.080 5.0 0 0.000 0.0 
200         
 100 7 0.070 6.0 2 0.020 2.0 5 0.050 5.0 0 0.000 0.0 
300         
 100 6 0.060 6.0 1 0.010 1.0 5 0.050 5.0 0 0.000 0.0 
400         
 100 7 0.070 7.0 2 0.020 2.0 5 0.050 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 25 0.250 21.0 7 0.070 6.0 18 0.180 16.0 0 0.000 0.0
Trend: 0.381 -0.006 0.307  
Probability: 0.352 0.503 0.379  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 02/24/1982    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Water 
0         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: C.I. Direct brown 95 
38         
 100 8 0.080 8.0 1 0.010 1.0 7 0.070 7.0 0 0.000 0.0 
120         
 100 7 0.070 6.0 2 0.020 2.0 5 0.050 5.0 0 0.000 0.0 
380         
 100 8 0.080 8.0 3 0.030 3.0 5 0.050 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 12 0.120 12.0 3 0.030 3.0 9 0.090 9.0 0 0.000 0.0
Trend: 0.907 1.840 0.100  
Probability: 0.182 0.033 0.460